SAB4200277

Anti-RCC1 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonyme(s) : Anti-CHC-1, Anti-CHC1, Anti-Chromosome Condensation 1, Anti-Regulator of Chromatin Condensation 1
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: ~45 kDa
• Espèces réactives: human
• Concentration: ~1.0 mg/mL
• Technique(s): immunoprecipitation (IP): 2.5-5.0 μg using lysates of human HEK-293 cells.; indirect immunofluorescence: 1.0-2.0 μg/mL using paraformaldehyde fixed HeLa cells.; western blot: 0.5-1.0 μg/mL using lysates of HeLa cells
• Numéro d'accès UniProt: P18754
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... RCC1(1104)

Description

Description générale

Regulator of chromosome condensation 1 (RCC1) is a nuclear chromatin-bound protein and a guanine nucleotide exchange factor (GEF) for the nuclear Ras-related nuclear (Ran) protein. It consists of seven homologous repeats of 51−68 amino acid residues termed RCC1-like domains (RLDs), which adopt a seven-bladed β-propeller fold 2 and an amino terminal tail extension. The RCC1 gene is mapped to human chromosome 1p35.3.(4)

Spécificité

Anti-RCC1 (N-terminal) recognizes human RCC1.

Immunogène

synthetic peptide corresponding to a sequence at the N- terminal region of human RCC1, conjugated to KLH. The corresponding sequence differs by one amino acid in rat or mouse.

Application

Anti-RCC1 (N-terminal) antibody produced in rabbit is suitable for:

• immunoblotting
• immunofluorescence
• immunoprecipitation

Actions biochimiques/physiologiques

Regulator of chromosome condensation 1 (RCC1) protein is an important cell cycle regulator. It is associated dynamically with chromatin through binding to histones H2A and/or H2B in a Ras-related nuclear (Ran) regulated manner. RCC1 has been shown to play essential roles in nuclear transport, spindle organization and nuclear envelop formation. α-N-methylation of RCC1 is essential for strong attachment of RCC1 to chromatin during normal mitosis.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable