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S9194

Sigma-Aldrich

SYBR® Green JumpStart™ Taq ReadyMix™ for High Throughput qPCR

SYBR® Green qPCR reagent, passive reference dye included

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About This Item

Code UNSPSC :
41106300
Nomenclature NACRES :
NA.55

Niveau de qualité

200

Forme

liquid

Utilisation

sufficient for 20 reactions
 sufficient for 2000 reactions
 sufficient for 400 reactions

Caractéristiques

dNTPs included
hotstart

Concentration

1.25 units/reaction (50 μL reaction volume)

Technique(s)

qPCR: suitable

Couleur

colorless

Entrée

purified DNA

Compatibilité

for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus

Méthode de détection

SYBR® Green

Conditions d'expédition

wet ice

Température de stockage

−20°C

Catégories apparentées

Description générale

SYBR® Green JumpStart™ Taq ReadyMix™ is a ready-to-use 2X master mix that contains SYBR® Green I, JumpStart™ Taq DNA polymerase, 99% pure deoxynucleotides (dNTPs), and reaction buffer making it the ideal solution for performing high-throughput quantitative PCR. SYBR® Green I dye binds to and detects any dsDNA generated during amplification. The JumpStart™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.

Application

SYBR® Green JumpStart™ Taq ReadyMix™ for High Throughput qPCR has been used for the amplification and quantification of transcripts to analyze gene expression in 2-step quantitative reverse transcription PCR (RT-qPCR). It has also been used for quantitative polymerase chain reaction (qPCR) of reverse-transcribed cDNA.

Caractéristiques et avantages

  • The master mix allows consistency and reproducibility from one reaction to the next
  • Reduced set-up time as compared to manual or wax Hot Start methods
  • Reduced primer dimers
  • Allows for room temperature set-up; Ideal for high throughput, quantitative PCR application
  • SYBR® Green I dye binds to double-stranded DNA and is ideal for quantifying any DNA sequence. Detection is monitored by measuring the increase in fluorescence throughout cycling.
  • JumpStart Taq DNA polymerase prevents amplification of non-specific products while increasing target yield.
  • Internal Reference Dye is provided for reaction normalization. Maximum excitation and emission is 586 nm and 605 nm, respectively.
  • SYBR Green JumpStart Taq ReadyMix reduces preparation time and the risk of contamination from multiple pipetting steps.

Conditionnement

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
400RXN is packaged as 1 X 10 mL
2000RXN is packaged as 1 X 50 mL

Autres remarques

SYBR Green ReadyMix for High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq and SYBR Green I in an easy-to-use ReadyMix solution that incorporates ROX dye for ABI and other real time instrument applications. The ReadyMix includes a detection fluor, internal standard and reagents for PCR making it the ideal solution for performing high-throughput quantitative PCR.

Informations légales

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

Produit(s) apparenté(s)

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Description
Tarif

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Eau, PCR Reagent

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SYBR® Green JumpStart™ Taq ReadyMix™ for Quantitative PCR, Capillary Formulation, SYBR® Green qPCR reagent for Roche LightCycler® capillary systems

S5193

JumpStart™ Taq ReadyMix avec SYBR® Green™, for quantitative PCR, without MgCl2 in buffer

L6544

LuminoCt® SYBR® Green qPCR ReadyMix™, For fast SYBR Green quantitative PCR

KCQS00

KiCqStart® SYBR® Green qPCR ReadyMix™, For Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems

KCQS01

KiCqStart® SYBR® Green qPCR ReadyMix™, Low ROX™, for ABI and Stratagene instruments

KCQS02

KiCqStart® SYBR® Green qPCR ReadyMix™, with ROX™ for ABI instruments

KCQS03

KiCqStart® SYBR® Green qPCR ReadyMix™, iQ™, with fluorescein for Bio-Rad systems

Pictogrammes

Exclamation markEnvironment
GHS07,GHS09

Mention d'avertissement

Warning

Mentions de danger

H315,H317,H319,H411

Classification des risques

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Gene resistance to transcriptional reprogramming following nuclear transfer is directly mediated by multiple chromatin-repressive pathways
<BIG><BIG>Jullien J, et al.</BIG></BIG>
Molecular Cell, 873-884 (2017)
Sambrook, J., and Russell, D.W.
Molecular Cloning: A Laboratory Manual null
Narcisa Muresu et al.
International journal of environmental research and public health, 17(12) (2020-06-27)
Objectives: Anal cancer is a rare disease. However, its incidence is increasing in some population groups. Infection caused by Human Papillomavirus (HPV) is strongly associated with the risk of anal cancer, whose variability depends on samples, histology, and HPV detection
Narcisa Muresu et al.
Virology journal, 17(1), 161-161 (2020-10-24)
Human Papillomavirus (HPV) infection is one of the most important causes of cancer. It can play a role in cervical and extra-cervical cancers. Penile cancer is rare, even if an increasing trend was recently reported. Aim of the present study
Fank1 and Jazf1 promote multiciliated cell differentiation in the mouse airway epithelium
<BIG><BIG>Johnson JA, et al.</BIG></BIG>
Biology Open, 7, bio033944-bio033944 (2018)
Afficher tous les articles scientifiques apparentés

Articles

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Protocoles

SYBR® Green I Dye Quantitative PCR Protocol

A protocol that can be used as a basic template for qPCR incorporating SYBR Green I DNA binding dye that is amenable to modification and applicable for use as validation for a set of primers.

Universal SYBR Green qPCR Protocol

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

Protocole de qPCR universel avec SYBR Green

Notre protocole de qPCR avec SYBR Green est conçu pour quantifier précisément l'expression génique ou des réactions de RT-PCR.

Contenu apparenté

SYBR® Green Based Quantitative PCR

SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR

RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR detects specific targets with applications in gene expression and pathogen detection.

RT-qPCR (PCR quantitative après transcription inverse)

La RT-qPCR, ou PCR quantitative après transcription inverse, combine les effets de la transcription inverse et de la PCR quantitative, ou PCR en temps réel, pour amplifier et détecter des cibles spécifiques. La RT-qPCR trouve de multiples applications, notamment dans la quantification du niveau d'expression génique, la validation de l'interférence par ARN ou la détection d'agents pathogènes comme les virus.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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