S5069

Anti-Superoxide Dismutase (MnSOD) (DD-17) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-IPO-B, Anti-Indophenoloxidase B, Anti-MNSOD, Anti-Manganese Dependent SOD, Anti-Manganese Superoxide Dismutase 2, Anti-Mitochodrial SOD, Anti-Mn-SOD, Anti-SOD2, Anti-Superoxide Dismutase 2
• Numéro MDL: MFCD06798545
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~24 kDa
• Espèces réactives: rat, human
• Validation améliorée: independent; Learn more about Antibody Enhanced Validation
• Technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using biotin/ExtraAvidin^®-peroxidase staining of human heart sections; indirect immunofluorescence: 10-20 μg/mL using methanol-acetone-fixed rat NRK cells; western blot: 1-2 μg/mL using human HeLa mitochondria extract and a chemiluminescent detection reagent; western blot: 2-4 μg/mL using whole extract of rat brain and a chemiluminescent detection reagent
• Numéro d'accès UniProt: P04179
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SOD2(6648) ; rat ... Sod2(24787)

Description

Description générale

Superoxide Dismutase (SOD) is a metalloprotein enzyme. It is widely distributed in the animal and plant kingdoms. The three common mammalian endogenous isozymes are the homodimeric copper-zinc SOD (Cu,ZnSOD, SOD1) found primarily in the cytosol, the homotetrameric glycosylated copper-zinc SOD, extracellular SOD (EC-SOD, SOD3), and the homotetrameric mitochondrial manganese SOD (MnSOD, SOD2). All three are nuclear-encoded. MnSOD is composed of four subunits, each containing one Mn²⁺.

Immunogène

synthetic peptide corresponding to amino acid residues 183-199 of human superoxide dismutase (MnSOD) with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is conserved in many eukaryotes.

Application

The antibody may be detected by various immunochemical techniques including immunoblotting, co-immunoprecipitation assay (co-IP), immunoprecipitation, indirect immunofluorescence and immunohistochemistry.

Actions biochimiques/physiologiques

Superoxide Dismutase (SOD) catalyzes the dismutation of the cytotoxic superoxide radical (O² -) to molecular oxygen and hydrogen peroxide. Manganese SOD (MnSOD) has been proposed to function as a tumor suppressor by modulating the activity of redox-sensitive transcription factors and specific signal mediators. MnSOD is involved in detoxification.

Forme physique

Solution in phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Informations légales

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable