Following disruption using Product No. RTN9604 - GenElute™ 96 Well Total RNA Purification Kit, the samples can be stored at -70°C in Lysis Buffer. Frozen samples are stable for up to 6 months. Before commencing the RNA isolation, frozen samples in Lysis Buffer should be completely thawed and centrifuged.
RTN9604
GenElute™ 96 Well Total RNA Purification Kit
Alternative plus écologique
4 x 96 well plate, sufficient for 4 96-well plate purifications
Synonyme(s) :
High Throughput Purification, RNA Purification
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| À vous/Référence | Disponibilité | Prix |
|---|---|---|
1 kit | Vérifier la disponibilité des articles du panier | 1 960,00 € |
A propos de cet article
NACRES:
NA.52
UNSPSC Code:
41105501
1 960,00 €
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sufficient for 4 96-well plate purifications
quality
4 x 96 well plate
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
RNA purification: suitable
input
mammalian cells
mammalian tissue
test parameters
: ~70 min kit run time per plate, sample volume: up to 10^7 cells or 30 mg of tissue
greener alternative category
storage temp.
15-25°C
General description
GenElute 96 Well Total RNA Purification Kit allows for the high-throughput purification of total RNA from cells or tissue. The kit utilizes the advantages of silica membrane technology and eliminates the use of hazardous organic compounds such as phenol and chloroform.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Application
The purified total RNA is ready for use in any enzymatic downstream application.
Biochem/physiol Actions
Sigma′s GenElute 96 Well Total RNA Purification Kit provides a simple and convenient way to isolate total RNA from cells or tissue. The cells or tissue are lysed in a solution containing large amounts of chaotropic salt which immediately inactivates RNases that are present in almost all biological materials. The addition of a Wash Buffer provides the necessary binding conditions for the RNA to be adsorbed to the silica membrane. The contaminating DNA which may be bound to the silica membrane is removed by directly applying RNase-free DNase. The remaining salts, proteins, and other cellular debris are removed by additional washing steps. The final step is to elute the purified RNA in the RNAase-free water that is supplied in the kit.
Features and Benefits
- Unique plate design offers low risk of cross-contamination
- RNA can be used for any kind of downstream enzymatic reaction
- Processing possible under with a vacuum or centrifuge
- Suitable for manual and automated processing
Other Notes
Application notes:
Method of purification:Silica-membrane technology
Compatible with Manual or automated, vacuum or centrifugation
Efficient recovery of > 200 nt
Typical yield20 μg (2 x 106 HeLa cells),
20 μg (20 mg mouse liver)
Typical concentration50–200 ng/μL
Elution volume50–130 μL
Preparation time70 min/plate
Binding capacity100 μg
RNA ratio28S/18S approx. 2.1
A260/A2801.90–2.10
Typical RIN (RNA > 9 (cells)
integrity number)≥ 7 (tissue)
Sufficient for:
< 107 cultured cells (centrifugation)
< 30 mg tissue (centrifugation)
saliva
Related products:
Prod #Related prod #
RTN9604RTN9602
RTN9602RTN9604
Method of purification:Silica-membrane technology
Compatible with Manual or automated, vacuum or centrifugation
Efficient recovery of > 200 nt
Typical yield20 μg (2 x 106 HeLa cells),
20 μg (20 mg mouse liver)
Typical concentration50–200 ng/μL
Elution volume50–130 μL
Preparation time70 min/plate
Binding capacity100 μg
RNA ratio28S/18S approx. 2.1
A260/A2801.90–2.10
Typical RIN (RNA > 9 (cells)
integrity number)≥ 7 (tissue)
Sufficient for:
< 107 cultured cells (centrifugation)
< 30 mg tissue (centrifugation)
saliva
Related products:
Prod #Related prod #
RTN9604RTN9602
RTN9602RTN9604
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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Cet article | |||
|---|---|---|---|
| description 4 x 96 well plate, sufficient for 4 96-well plate purifications | description 4 x 96 well plate, sufficient for 4 96-well plate purifications | description sufficient for 10 purifications | description sufficient for 70 purifications |
| usage sufficient for 4 96-well plate purifications | usage sufficient for 4 96-well plate purifications | usage sufficient for 10 purifications | usage sufficient for 70 purifications |
| technique(s) RNA purification: suitable | technique(s) DNA purification: suitable | technique(s) RNA purification: suitable | technique(s) RNA purification: suitable |
| test parameters sample volume: up to 10^7 cells or 30 mg of tissue, : ~70 min kit run time per plate | test parameters sample volume: 100 μL, : <45 min kit run time | test parameters : <30 min kit run time, sample volume: up to 10^7 cells or 40 mg of tissue | test parameters sample volume: up to 10^7 cells or 40 mg of tissue, : <30 min kit run time |
| input mammalian cells | input sample(s) (PCR) | input mammalian cells | input mammalian tissue |
| greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics - | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals |
| greener alternative category | greener alternative category - | greener alternative category , Aligned | greener alternative category |
Composants de kit seuls
Réf. du produit
Description
- RNA Wash Buffer 2, Concentrate
signalword
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1C - Skin Sens. 1
supp_hazards
Classe de stockage
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
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Can the lysates, after the cells are lysed in the kits lysis buffer, be stored at -80°C, or is it necessary for the RNA purification to be carried out immediately?
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