RAB0366

Mouse MMP-2 ELISA Kit

Name: Mouse MMP-2 ELISA Kit
Brand: SIGMA
Price: 549 EUR

for serum, plasma and cell culture supernatant

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A propos de cet article

NACRES:

NA.32

UNSPSC Code:

41116158

Gene information:

mouse ... Mmp2(17390)

Input:

sample type plasma
sample type serum
sample type cell culture supernatant(s)

Species reactivity:

mouse

Storage temp.:

−20°C

Shipped in:

wet ice

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species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 27.43-20000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Mmp2(17390)

Catégories apparentées

Enzyme-Linked Immunosorbent Assay (ELISA) Kits

Immunoassay Platform Solutions

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Cet article	RAB0373	RAB0368	RAB0367
Sigma-Aldrich RAB0366 Mouse MMP-2 ELISA Kit	Sigma-Aldrich RAB0373 Mouse pro-MMP-9 ELISA Kit	Sigma-Aldrich RAB0368 Mouse MMP-3 ELISA Kit	Sigma-Aldrich RAB0367 Human MMP-3 ELISA Kit
species reactivity mouse	species reactivity mouse	species reactivity mouse	species reactivity human
Gene Information mouse ... Mmp2(17390)	Gene Information mouse ... Mmp9(17395)	Gene Information mouse ... Mmp3(17392)	Gene Information human ... MMP3(185250)
input sample type plasma sample type serum sample type cell culture supernatant(s)	input sample type plasma sample type cell culture supernatant(s) sample type serum	input sample type serum sample type cell culture supernatant(s) sample type plasma	input sample type cell culture supernatant(s) sample type urine sample type serum sample type plasma
assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 25 pg/mL standard curve range: 27.43-20000 pg/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 50 pg/mL standard curve range: 74.07-18000 pg/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 10 pg/mL standard curve range: 13.72-10000 pg/mL	assay range inter-assay cv: <12% intra-assay cv: <10% sensitivity: 300 pg/mL standard curve range: 0.41-100 ng/mL
technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable	technique(s) ELISA: suitable, capture ELISA: suitable
detection method colorimetric	detection method colorimetric	detection method colorimetric	detection method colorimetric

General description

Matrix Metallopeptidase 2 (MMP-2), also known as gelatinase A, belongs to the gelatinase subclass of the matrix metalloproteinase (MMP) family. It contains zinc in the active site.[1] MMP-2 cleaves gelatin, type IV, V, VII, X, and XI collagens, fibronectin, vitronectin, elastin, laminin, proteoglycans, and a range of non-extracellular matrix (ECM) components.[2][1] Both MMP-2 and MMP9 are important for matrix degradation and they are implicated in the maintenance of neovascularization.[3] The Mouse MMP-2 enzyme-linked immunosorbent assay (ELISA) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MMP-2 in serum, plasma, cell culture supernatants, and urine.

Immunogen

Recombinant Mouse MMP2

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Mouse MMP-2 ELISA Kit has been used in enzyme-linked immunosorbent assay to quantify matrix metalloproteinase -2 (MMP-2) secreted in supernatants by gold nanoparticles (AuNP)-loaded endothelial cells[4] and superparamagnetic iron oxide nanoparticle (SPION) loaded endothelial cells.[5]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Composants de kit également disponibles séparément

Réf. du produit

Description

FDS

RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)FDS

RABSTOP3ELISA Stop Solution (Item I)FDS

RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)FDS

RABWASH420X Wash Buffer (Item B)FDS

pictograms

GHS05

signalword

Warning

hcodes

H290

pcodes

P234 - P390

Hazard Classifications

Met. Corr. 1

Classe de stockage

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Disturbance of adhesomes by gold nanoparticles reveals a size- and cell type-bias.

Mulens-Arias, et al.

Biomaterials Science, 7, 389-408 (2019)

Matrix metalloproteinase-2 (MMP-2) and MMP-9 in pulmonary pathology.

Subhadeep Chakrabarti et al.

Experimental lung research, 31(6), 599-621 (2005-07-16)

The lung is affected by a variety of disease processes that can lead to considerable morbidity and mortality. As the lung is the only organ for respiration and gas exchange, the structural and functional integrity of the lung is of

Theranostic iron oxide nanoparticle cargo defines extracellular vesicle-dependent modulation of macrophage activation and migratory behavior

Mulens-Arias, V, et al.

Advanced Biosystems, 1800079-1800079 (2018)

Matrix metalloproteinases, a disintegrin and metalloproteinases, and a disintegrin and metalloproteinases with thrombospondin motifs in non-neoplastic diseases.

Takayuki Shiomi et al.

Pathology international, 60(7), 477-496 (2010-07-03)

Cellular functions within tissues are strictly regulated by the tissue microenvironment which comprises extracellular matrix and extracellular matrix-deposited factors such as growth factors, cytokines and chemokines. These molecules are metabolized by matrix metalloproteinases (MMP), a disintegrin and metalloproteinases (ADAM) and

Thrombospondin-1 suppresses spontaneous tumor growth and inhibits activation of matrix metalloproteinase-9 and mobilization of vascular endothelial growth factor.

J C Rodriguez-Manzaneque et al.

Proceedings of the National Academy of Sciences of the United States of America, 98(22), 12485-12490 (2001-10-19)

Growth of tumors and metastasis are processes known to require neovascularization. To ascertain the participation of the endogenous angiogenic inhibitor thrombospondin-1 (TSP1) in tumor progression, we generated mammary tumor-prone mice that either lack, or specifically overexpress, TSP1 in the mammary

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Contenu apparenté

ELISA Kit Information

Instructions

User Guide-RAB0366

Questions

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