Accéder au contenu
Merck
Toutes les photos(2)

Documents

RAB0145

Sigma-Aldrich

Human Dkk-3 ELISA Kit

for serum, plasma, cell culture supernatants and urine

Synonyme(s) :

Dickkopf-related protein 3, Dkk-3

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(2)

About This Item

Code UNSPSC :
41116158
Nomenclature NACRES :
NA.32

Espèces réactives

human

Conditionnement

kit of 96 wells (12 strips x 8 wells)

Technique(s)

ELISA: suitable
capture ELISA: suitable

Entrée

sample type cell culture supernatant(s)
sample type plasma
sample type urine
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 2.74-2000 pg/mL

Méthode de détection

colorimetric

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... DKK3(27122)

Description générale

The Human Dickkopf homolog 3 (DKK3) enzyme-linked immunosorbent assay (ELISA) kit is an in vitro enzyme-linked immunosorbent assay used for the quantitative measurement of human DKK-3 in serum, plasma, cell culture supernatants, and urine. Dickkopf homolog 3 (DKK3) protein belongs to the Dkk family. The DKK3 gene acts as a tumor suppressor gene and is suppressed in several human cancers. This gene is mapped on the human chromosome at 11p15.3.

Immunogène

Recombinant Human DKK3

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Dkk-3 ELISA Kit has been used to evaluate Dickkopf homolog 3 (DKK3) from conditioned media. It has also been used to determine DKK3 levels in urine samples.

Autres remarques

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)FDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)FDS
  • RABSTOP3ELISA Stop Solution (Item I)FDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)FDS
  • RABWASH420X Wash Buffer (Item B)FDS

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Warning

Mentions de danger

H290

Conseils de prudence

P234 - P390

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Francesca Valdora et al.
International journal of molecular sciences, 14(4), 7492-7505 (2013-04-10)
Medulloblastoma (MB) is a malignant pediatric brain tumor arising in the cerebellum consisting of four distinct subgroups: WNT, SHH, Group 3 and Group 4, which exhibit different molecular phenotypes. We studied the expression of Dickkopf (DKK) 1-4 family genes, inhibitors
Kazuyasu Kobayashi et al.
Gene, 282(1-2), 151-158 (2002-01-30)
The human REIC gene is a recently found mortalization-related gene and a candidate tumor suppressor gene expression of which is largely attenuated in many immortalized and tumor-derived cell lines (Biochem. Biophys. Res. Commun. 268 (2000) 20-24). To gain insight into
Kenta Torigoe et al.
Medicina (Kaunas, Lithuania), 57(6) (2021-07-03)
Background and Objectives: Urinary levels of dickkopf-3 (DKK-3) are associated with poor renal survival in patients with non-dialytic chronic kidney disease. However, it remains unknown whether urinary DKK-3 levels can predict residual renal function (RRF) decline in patients undergoing peritoneal
Jun Yu et al.
Cancer, 115(1), 49-60 (2008-12-04)
Abnormal activation of the Wnt/beta-catenin signaling pathway is common and critical in the pathogenesis of digestive cancers. In this study, the authors investigated the promoter methylation of the dickkopf homolog 3 gene Dkk-3 in these cancers and its prognostic significance
Shuang Zhao et al.
Frontiers in oncology, 10, 600322-600322 (2021-01-12)
Dkk3 has been discovered during comparison of immortalized and parental cells. Its expression has been shown to reduce colony formation and induce apoptosis of cancer cells, acting as a tumor suppressor. Herein, we demonstrate that Dkk3 overexpression or protein treatment
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique