R0260
BamH I from Bacillus amyloliquefaciens H
Restriction Enzyme
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About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352204
Produits recommandés
Qualité
for molecular biology
Forme
buffered aqueous glycerol solution
Concentration
10,000 units/mL
Conditions d'expédition
wet ice
Température de stockage
−20°C
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Spécificité
Recognition sequence: 5′-G/GATCC-3′
Cutting results: a 2-10-fold Bam HI overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 60 °C for 15 minutes.
Star activity: To prevent star activity, avoid suboptimal reaction conditions containing low salt concentration, high glycerol (>5%) and high pH 8.0.
Cutting results: a 2-10-fold Bam HI overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 60 °C for 15 minutes.
Star activity: To prevent star activity, avoid suboptimal reaction conditions containing low salt concentration, high glycerol (>5%) and high pH 8.0.
Application
BamHI is a DNA restriction endonuclease that is used in molecular biology applications to cleave DNA at the recognition sequence 5′-G/GATCC-3′ to generate 5′-cohesive termini.
Autres remarques
Supplied with 10x Restriction Enzyme Buffer SB (B8781).
Forme physique
Solution in 10 mM Tris-HCl, pH 7.4 , 1 mM EDTA, 1mM dithioerythritol, 300 mM KCl, 0.01% Polydocanol (v/v), 50% glycerol (v/v), at 4°C
Produit(s) apparenté(s)
Tampon d'incubation
Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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J H Ellis et al.
Journal of immunology (Baltimore, Md. : 1950), 156(8), 2700-2709 (1996-04-15)
CD80 and CD86 are cell surface glycoproteins expressed on a variety of professional APCs. They have attracted much attention due to their function as potent costimulators of T lymphocyte function through their interaction with CD28 and possibly CTLA4. Because inhibitors
Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H.
G A Wilson et al.
Journal of molecular biology, 97(1), 123-125 (1975-09-05)
P Manivasakam et al.
Nucleic acids research, 29(23), 4826-4833 (2001-12-01)
Mammalian cells repair DNA double-strand breaks by illegitimate end-joining or by homologous recombination. We investigated the effects of restriction enzymes on illegitimate and homologous DNA integration in mammalian cells. A plasmid containing the neo(R) expression cassette, which confers G418 resistance
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis
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