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PP0100

Sigma-Aldrich

Trypsin Profile IGD Kit

Synonyme(s) :

In gel digestion kit, Proteomics grade Trypsin, TPCK treated

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1 KIT
481,00 €

481,00 €

Date d'expédition estimée le15 avril 2025

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1 KIT
481,00 €

About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.56

481,00 €

Date d'expédition estimée le15 avril 2025

Devis pour commande en gros

Utilisation

 kit sufficient for ≤100 applications

Niveau de qualité

200

Température de stockage

2-8°C

Description générale

The Trypsin Profile IGD Kit enables fast, efficient, and complete in-gel tryptic digestion of up to 100 excised protein spots. Digested proteins are ready for MALDI-MS (matrix-assisted laser desorption/ionization-mass spectrometry) and require no additional preparation. Because the Trypsin Profile IGD Kit contains Proteomics Grade Trypsin, a higher sequence coverage and fewer ambiguous autolytic peaks are observed in MALDI spectra.
Trypsin has been chemically modified through reductive methylation of the ε-amino groups of lysine to reduce autolysis and minimize autolytic fragments. In addition, it has been TPCK (tosyl phenylalanyl chloromethyl ketone) treated to remove residual chymotrypsin activity and then further purified by affinity chromatography, yielding a highly purified trypsin suitable for proteomics work.

Application

Trypsin Profile IGD Kit has been used for in-gel digestion for LC-MS (liquid chromatography-mass spectrometry)/MS and MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass spectrometry analysis.[1][2]

Caractéristiques et avantages

  • Faster destaining than with alternative in-gel digest kits
  • Includes 100% of the reagents needed to destain, digest, and extract proteins/peptides of interest
  • Compatible with Coomassie, SYPRO® Orange, SYPRO Ruby, and ProteoSilver™ Stained gels
  • Resulting samples are ready for analysis by MALDI-MS or HPLC-MS

Informations légales

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC
SYPRO is a registered trademark of Life Technologies

Composants de kit seuls

Réf. du produit
Description
  • Destaining Solution Reconstituted to 75

Pictogrammes

FlameHealth hazardExclamation mark
GHS02,GHS08,GHS07

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

3 - Flammable liquids

Point d'éclair (°F)

35.6 °F - closed cup

Point d'éclair (°C)

2.0 °C - closed cup

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
SLCQ8498
SLCJ8802
SLCD4484
SLCB1308
SLBW2626

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

X-M Lu et al.
International journal of burns and trauma, 3(1), 37-48 (2013-02-07)
Insulin resistance is a major effect of burn injury and insulin receptor substrate-1 (IRS-1) plays an important role in signal transduction. Here, we explored the integrity of IRS-1 in muscle after burn injury. A murine model of severe burn injury
Shuo Han et al.
Cell death & disease, 12(11), 1070-1070 (2021-11-12)
Uncontrolled overactivation of autophagy may lead to autophagic cell death, suppression of which is a pro-survival strategy for tumors. However, mechanisms involving key regulators in modulating autophagic cell death remain poorly defined. Here, we report a novel long noncoding RNA
Xingyuan Liu et al.
Cell death & disease, 13(7), 642-642 (2022-07-24)
Hepatocellular carcinoma (HCC) is the most common subtype of liver cancer and the second most fatal cancer in the world despite the great therapeutic advances in the past two decades, which reminds us of the gap in fully understanding the
Identification of plant viruses using one-dimensional gel electrophoresis and peptide mass fingerprints.
Luo, H., et al.
J. Virol. Methods, 165(2), 297-301 (2010)
SILAM for quantitative proteomics of liver Akt1/PKBa after burn injury.
Lu XM, et al.
International Journal of Molecular Medicine, 29, 461-461 (2012)
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