The PKH cell linker kits have not been specifically tested for the detection of circulatory fibrocytes. However, there are several publications that indicate suitability. Please see the link below to review one such reference:
https://onlinelibrary.wiley.com/doi/full/10.1111/jcmm.13080
PKH26PCL
PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling
Distributed for Phanos Technologies
Synonyme(s) :
Phagocytic cell label
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1 KIT
1 670,00 €
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Changer de vue
1 KIT
1 670,00 €
About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.32
1 670,00 €
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Niveau de qualité
Application
This kit is for phagocytic cell labeling. It is used to selectively label cells with phagocytic capabilities such as monocytes, macrophages or neutrophils.
Principe
The labeling occurs through the formation of dye aggregates or particulates. The aggregate formation significantly inhibits the uptake of dye by non-phagocytic cells, such as lymphocytes, but facilitates dye uptake by phagocytic cells. Labeled cells appear patchy or spotted because the dye is localized in phagocytic compartments of the cells. The dye appears to be resistant to metabolic attack and has been found to remain with the cells for at least 21 days in vivo.
Labeling of phagocytic cells by this methodology may be conducted either in vitro or in vivo. Intraperitoneal or intravenous injections of the PKH26 labeling solution will successfully label phagocytic cells in vivo, while cells of interest which have been isolated may be stained using in vitro labeling methods.
Labeling of phagocytic cells by this methodology may be conducted either in vitro or in vivo. Intraperitoneal or intravenous injections of the PKH26 labeling solution will successfully label phagocytic cells in vivo, while cells of interest which have been isolated may be stained using in vitro labeling methods.
Liaison
For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.
Informations légales
CellVue is a registered trademark of Phanos Technologies
Composants de kit seuls
Réf. du produit
Description
- Diluent B 6 x 10
- PKH26 cell linker in ethanol .5 mL
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Flam. Liq. 2
Code de la classe de stockage
3 - Flammable liquids
Point d'éclair (°F)
57.2 °F - closed cup
Point d'éclair (°C)
14.0 °C - closed cup
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Articles
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PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.
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Is this kit suitable for label circulatory fibrocyte isolated from rat PBMC?
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