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Sigma-Aldrich

TruPAGE™ Precast Gels

4-20%, 10 x 8cm, 12-well

Synonyme(s) :

Precast Gels for SDS-PAGE, Precast Polyacrylamide Gels

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About This Item

Code UNSPSC :
41105300
Nomenclature NACRES :
NB.22

Description

for use with Bio-Rad Mini-PROTEAN™ Tetra Cell, Mini-PROTEAN Dodeca™, Mini-PROTEAN II and 3 cells reverse device gasket to flat side

Durée de conservation

24 mo. at 2‑8 °C (from the date of manufacture)

Conditionnement

box of 10 ea

Concentration

4-20% polyacrylamide

Cassette, l × L

10 cm × 8 cm

Épaisseur du gel

1 mm

Taille des puits × volume

12 wells × 35 μL

Compatibilité

for use with Hoefer Mighty Small II and Tall Mighty Small (SE 260 and SE280)

Température de stockage

2-8°C

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Caractéristiques et avantages

TruPAGE SDS-PAGE Precast Gels provide improved protein resolution, tear resistance, and an extended shelf-life. TruPAGE gels are formulated with Triethanolamine (TEA)-Tricine, featuring a neutral pH running environment that helps prevents protein modification during the course of the experiment.
The precast gels for SDS-PAGE come in two gel cassette sizes (10 x 10 cm and 10 x 8 cm) for increased equipment compatibility. TruPAGE gels have extra tall wells to prevent lane-to-lane overflow and come in two well formats (12-well and 17-well). TruPAGE gels are available in several different gel concentrations (10%, 12%, 4-8%, 4-12% and 4-20%) to provide desired resolution of proteins of any size. Please refer to the migration chart to select the appropriate precast gel and running buffer combination for your experimental needs. Only use TruPAGE formulated buffers when running TruPAGE gels to ensure optimal results and reproducibility. Please review the TruPAGE Technical Bulletin for buffer formulation recipes and general running guidelines.

  • Long Shelf Life - 2 years from the date of manufacture when stored properly.
  • High Performance - Extended full length resoling gels provides improved resolution.
  • Durable - Stronger than standard polyacrylamide gels, preventing tearing during post-electrophoresis processing.
  • Extra Tall Wells - Eliminate sample spill over and accommodate larger sample loading volumes with well teeth that protrude above the back plate of the gel cassette.
  • Excellent Compatibility - TruPAGE gels are compatible with the popular gel running equipment, including Sigma-Aldrich′s Dual Run Tank (Z741768) and other manufacturers like Life Technologies, Bio-Rad®, Lonza®, Hoefer®, CBS Scientific as well as others, allowing you to continue using the equipment you already own.
  • Fast - Run times as low as 30 minutes.
  • Great Value - Gels and buffers offer a cost-saving alternative without sacrificing performance and experimental integrity.
TruPAGE Precast Gels Migration Chart
There is no tape to remove. There is also no comb to remove, which prevents torn or bent wells. Instead, the cassettes lock well fingers in place.
TruPAGE™ Precast Gels 4-20% have been used to separate proteins through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by Western blotting.

Informations légales

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.
Hoefer is a registered trademark of Hoefer, Inc.
Lonza is a registered trademark of Lonza Group Ltd.
TruPAGE is a trademark of Sigma-Aldrich Co. LLC

Configuré(e)(s) pour

Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Registered report: Transcriptional amplification in tumor cells with elevated c-Myc.
Blum D, et al.
eLife, 4, e04024-e04024 (2015)
Amanda L Wong et al.
Methods (San Diego, Calif.), 184, 102-111 (2020-02-06)
The development of universal, broadly applicable methods for histone extraction from animal cells and tissues has unlocked the ability to compare these epigenetic-influencing proteins across tissue types, healthy and diseased states, and cancerous versus normal cells. However, for plants and
Camila Yattah et al.
Neurochemical research, 45(3), 606-619 (2020-02-06)
Differentiation of oligodendrocytes (OL) from progenitor cells (OPC) is the result of a unique program of gene expression, which is further regulated by the formation of topological domains of association with the nuclear lamina. In this study, we show that

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