P5306
Papaïne from papaya latex
lyophilized powder, aseptically filled
Synonyme(s) :
Papaïnase
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About This Item
Produits recommandés
Source biologique
papaya (latex)
Niveau de qualité
Stérilité
aseptically filled
Forme
lyophilized powder
Activité spécifique
≥8.0 units/mg protein
Poids mol.
23.4 kDa
Solubilité
H2O: soluble 1.2 mg/mL
Température de stockage
−20°C
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Description générale
Papain is a thermostable enzyme and becomes inactive at 55°C. It has two domains and the catalytic region is present at the domain interface. The cysteine 25 in the active site performs the proteolysis by nucleophilic attack on the carbonyl carbon of a peptides. Papain has wide industrial applications in food and pharmaceutical industry.
Application
Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies . It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P5306 is provided as a lyophilized powder that is aseptically filled. It has been used to digest mouse retinas to study the RAGE axis in nonproliferative diabetic retinopathy (NPDR).
The product from Sigma has been used in the digestion of extracellular matrix (ECM). It has also been used with other proteolytic enzymes for in situ protein degradation to produce crystals suitable for structure determination.
Sert à produire des fragments Fab d′anticorps. Également utilisée pour la dissociation cellulaire, car des études ont montré qu′elle s′avère plus efficace et moins détériorante avec certains tissus.
Actions biochimiques/physiologiques
Papain papaya latex has antifungal activity against C. albicans. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
Protéase à cystéine qui clive les liaisons peptidiques des acides aminés basiques, de la leucine ou de la glycine.
pH optimal situé entre 6,0 et 7,0.
Hydrolyse également les esters et les amides.
pH optimal situé entre 6,0 et 7,0.
Hydrolyse également les esters et les amides.
Définition de l'unité
One unit will hydrolyze 1.0 μmole of BAEE per minute at pH 6.2 at 25 deg C.
Notes préparatoires
Prepared from P 4762
Autres remarques
Pour en savoir plus sur la papaïne, rendez-vous sur www.sigma-aldrich.com/enzymeexplorer.
Inhibiteur
Réf. du produit
Description
Tarif
Substrat
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.
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