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P4929

Sigma-Aldrich

Poly(deoxyinosinic-deoxycytidylic) acid sodium salt

double-stranded alternating copolymer

Synonyme(s) :

Poly(dI-dC) • Poly(dI-dC) sodium salt

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5 UNITS
280,00 €
10 UNITS
482,00 €
25 UNITS
960,00 €

280,00 €

Disponible pour expédition le07 avril 2025Détails

Devis pour commande en gros
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Sélectionner une taille de conditionnement

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5 UNITS
280,00 €
10 UNITS
482,00 €
25 UNITS
960,00 €

About This Item

Numéro CAS:
118578-37-3
Numéro MDL:
MFCD00131970
Code UNSPSC :
41106305
Nomenclature NACRES :
NA.51

280,00 €

Disponible pour expédition le07 avril 2025Détails

Devis pour commande en gros

Source biologique

synthetic (organic)

Niveau de qualité

200

Forme

powder

Température de stockage

−20°C

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Application

Poly(deoxyinosinic-deoxycytidylic) acid (Poly(dI-dC) • Poly(dI-dC)) is an alternating copolymer used as a DNA substrate for evaluation of DNA methytransfeases, such as DNA-methyltransferase 1 and as double-stranded DNA model for conformational studies of DNA structure dynamics and drug, small molecule, interactions.
Poly(deoxyinosinic-deoxycytidylic) acid sodium salt has been used in a study that synthesized and characterized bis(2-(pyrimidin-2-γl)ethoxy)alkanes and their pharmacological activity. Poly(deoxyinosinic-deoxycytidylic) acid sodium salt has also been used in a study that investigated the growth of calcium carbonate films on LB/LbL matrices.
Poly(deoxyinosinic-deoxycytidylic) acid sodium salt has been used in the electrophoretic mobility shift assay in p65 protein[1] and neuro-2A protein lysate.[2] It has also been used as a substrate in the protein arginine methyltransferase 1 (PRMT1) and DNA methyltransferase 1 (DNMT1) selectivity assay.[3]

Actions biochimiques/physiologiques

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid), poly(dI-dC) is a synthetic DNA substrate.[4] At high salt, Poly(dI-dC) exists in left handed helical conformation and reverts to right-handed form upon decreasing salt.[5] It is used in electrophoretic mobility shift assay (EMSA).[6]

Autres remarques

Double-stranded alternating copolymer

Définition de l'unité

One unit will yield an A260 of 1.0 in 1.0 ml of 20 mM sodium phosphate/100 mM NaCl, pH 7.0 (1 cm light path)

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000425632
0000425632
0000402757
0000389077
0000387689

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Chao Bi et al.
Plant molecular biology, 95(4-5), 425-439 (2017-09-20)
Nuclear factor Y (NF-Y) family proteins are involved in many developmental processes and responses to environmental cues in plants, but whether and how they regulate phytohormone abscisic acid (ABA) signaling need further studies. In the present study, we showed that
Identification of Selective, Cell Active Inhibitors of Protein Arginine Methyltransferase 5 through Structure-Based Virtual Screening and Biological Assays
Ye F, et al.
Journal of Chemical Information and Modeling, 58(5), 1066-1073 (2018)
Meaghan L Clark et al.
Inorganic chemistry, 47(20), 9410-9418 (2008-09-25)
This paper focuses on DNA-binding interactions exhibited by Pt(dma-T)CN(+), where dma-T denotes 4'-dimethylamino-2,2':6',2''-terpyridine, and includes complementary studies of the corresponding pyrr-T complex, where pyrr-T denotes 4'-(N-pyrrolidinyl)-2,2':6',2''-terpyridine. The chromophores are useful for understanding the interesting and rather intricate DNA-binding interactions exhibited
Chao Bi et al.
Plant molecular biology, 94(1-2), 197-213 (2017-04-10)
It has been known that ABA INSENSITIVE 5 (ABI5) plays a vital role in regulating seed germination. In the present study, we showed that inhibition of the catalase activity with 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of Col-0, abi5 mutants and
Dexamethasone treatment of calves latently infected with bovine herpesvirus 1 leads to activation of the bICP0 early promoter, in part by the cellular transcription factor C/EBP-alpha
Workman A, et al.
Journal of Virology, 83(17), 8800-8809 (2009)
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Questions

1–7 sur 7 questions  

  1. How to prepare the product of P4929-5U to 1ug/ul? What's the weight (mg), and molecular weight (g/mol) of this product?

    1 réponse

    1. The polymer size of this material varies from lot to lot. The typical low range is 150 to 550 base pairs and the typical high range is 3000 to 5000 base pairs. The bulk of the product ranges from 1200 to 3000 base pairs. The molecular weight of a single Poly(dI-dC) base pair is 639.41 g/mol, resulting in an approximate molecular weight range of 767.2 kDa – 1,918.2 kDa for most of the compound. The unit per milligram value may range from 8 – 14 units per milligram and the vial content may range from 5.0 – 7.5 units. The specific vial content is lot specific and may range from 535 – 625 ug per vial. To prepare a 1 ug/ul solution, please see the link below to review the lot specific Certificate of Analysis:
      https://www.sigmaaldrich.com/product/sigma/p4929#product-documentation

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  2. Should I heat the product P4929-25U after resuspension to make aliquots? If yes, then how long and at what temperature? Thank you!

    1 réponse

    1. This product is soluble in water or neutral buffer. Solubility is no longer considered part of the specifications, but it was previously reported to be soluble at 20 mg/mL in 0.1 M Tris-HCl, pH 8.8. Heat is not necessary to solubilize the product; dissolving in an appropriate solvent is the recommended method for resuspension.

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  3. What is the Department of Transportation shipping information for this product?

    1 réponse

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. Is Is Product P4929,  Poly(deoxyinosinic-deoxycytidylic) acid sodium salt (Poly(dI-dC), stable in solution?

    1 réponse

    1. Both the solution and solid are very stable and can be stored for extended periods (years) at -20 °C.

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  5. How many milligrams of Product P4929, Poly(deoxyinosinic-deoxycytidylic) acid sodium salt, do I have?

    1 réponse

    1. The specifications for the product is approximately 13 U/mg, but the exact amount is lot specific.  One unit will yield an A260 of 1.0 in 1.0ml of water.  For example, the 5 unit size has approximately 0.384 mg.

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  6. What is the molecular weight of Product P4929, Poly(deoxyinosinic-deoxycytidylic) acid sodium salt?

    1 réponse

    1. Molecular weight: Polymer size (by agarose gel electrophoresis) is lot dependent with a lower range of less than 150 to 550 base pairs and a high range of 3000 to over 5000 base pairs. Most of the material is between 1200 to 3000 base pairs.

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  7. What is the solubility of Is Product P4929,  Poly(deoxyinosinic-deoxycytidylic) acid sodium salt (Poly(dI-dC)?

    1 réponse

    1. This product is soluble in water or neutral buffer at 20 mg/ml.

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