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Principaux documents

P3543

Sigma-Aldrich

Poly-L-lysine–FITC Labeled

mol wt 15,000-30,000

Synonyme(s) :

Fluorescein Labeled Poly-L-Lysine

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About This Item

Numéro MDL:
MFCD00165427
Code UNSPSC :
12352209
Nomenclature NACRES :
NA.26
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Forme

powder

Niveau de qualité

200

Poids mol.

precursor poly-L-lysine • HBr 15,000-30,000
 15,000-30,000

Ampleur du marquage

0.003-0.01 mol FITC per mol lysine monomer

Couleur

yellow to orange

Température de stockage

−20°C

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Catégories apparentées

Autres remarques

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000334520
0000334507
0000321146
0000301871
0000258513

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Vitreous is a barrier in nonviral gene transfer by cationic lipids and polymers.
Pitkanen L, Ruponen M, et al.
Pharmacological Research, 20, 576-583 (2003)
Leena Pitkänen et al.
The AAPS journal, 6(3), e25-e25 (2005-03-12)
We investigated the permeation of liposomal and polymeric gene delivery systems through neural retina into retinal pigment epithelium (RPE) and determined the roles of various factors in permeation and subsequent uptake of the delivery systems by RPE. Anterior parts and
Larry J Millet et al.
Lab on a chip, 10(12), 1525-1535 (2010-04-15)
Wiring the nervous system relies on the interplay of intrinsic and extrinsic signaling molecules that control neurite extension, neuronal polarity, process maturation and experience-dependent refinement. Extrinsic signals establish and enrich neuron-neuron interactions during development. Understanding how such extrinsic cues direct
Marjo Männistö et al.
The journal of gene medicine, 7(4), 466-476 (2004-12-25)
Retinal pigment epithelium (RPE) maintains the function of photoreceptors and eyesight and is an important target for gene delivery. Since in some diseases RPE cells proliferate uncontrollably, we investigated the role of cell cycle in non-viral gene delivery into a

Articles

Poly(Amino Acid) Block Copolymers for Drug Delivery and other Biomedical Applications

Humankind has utilized protein materials throughout its existence, starting with the use of materials such as wool and silk for warmth and protection from the elements and continuing with the use of recombinant DNA techniques to synthesize proteins with unique and useful properties.

Questions

1–2 sur 2 questions  

  1. ow do you determine the peptide concentration of a small scoop in solution? I am reading the absorbance at 495nm to read the fluorophore but I don't know how to convert that Absorbance value to a meaningful mg/mL or molarity concentration without an e.c.

    1 réponse

    1. The viscosity average molecular weight (Mv) is determined for each lot of this product. However, there is a distribution of molecular weights around that Mv. As a result, the molar concentration is not applicable. The typical degree of polymerization (DP) for this material is approximately 100 and the typical degree of substitution (moles of FITC per mole of lysine) is approximately 0.007 or 7 per 1000; essentially 7 of 10 Poly-L-Lysine molecules contain one FITC label. The concentration of the solution may be most accurately determined by precisely weighing the sample and precisely determining the volume, so that the sample may be reported as mg/mL. The degree of substitution and degree of polymerization are lot specific and reported in the product Certificate of Analysis. Please see the link below to review a sample of lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/p3543#product-documentation

      Utile ?

  2. How much FITC quantity or weight percentage is present per mg of the mixture? If 10 mg of the product is weighed out, what portion of the weight corresponds to the PLL?

    1 réponse

    1. The FITC quantity present per mg of the mixture is 0.016 mg, which corresponds to approximately 1.6% of the weight. If 10 mg of the product is weighed out, the portion of the weight corresponding to the PLL is approximately 9.84 mg.

      Utile ?

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