MSP03
Membrane Scaffold Protein 1D1(-)
recombinant, expressed in E. coli, buffered aqueous solution
Synonyme(s) :
Membrane Scaffold Protein
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| À vous/Référence | Disponibilité | Prix |
|---|---|---|
5 mg | Vérifier la disponibilité des articles du panier | 508,00 € |
A propos de cet article
NACRES:
NA.26
UNSPSC Code:
12352200
Form:
buffered aqueous solution
Assay:
≥90% (SDS-GE)
Recombinant:
expressed in E. coli
Mol wt:
22,043.9 Da
Service technique
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Laissez-nous vous aiderrecombinant
expressed in E. coli
Quality Segment
assay
≥90% (SDS-GE)
form
buffered aqueous solution
mol wt
22,043.9 Da
solubility
water: soluble
shipped in
ambient
storage temp.
−20°C
General description
Research area: Cell structure. Membrane Scaffold Protein 1D1 (MSP1D1) is a variant of MSP1 where the first eleven amino acids in the helix 1 portion are deleted of the original MSP1 sequence. It consists of an N-terminal histidine-tagged protein with a tobacco etch virus (TEV) protease cleavage site that is present between the protein sequence and histidine-tag. MSP1D1(–)variant of MSP1D1 lacks the histidine-tag and the TEV protease-recognition sequence.
Application
For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.
Biochem/physiol Actions
Nano-disctechnology is an approach that allows aqueous soluble membrane proteins in anative-like bilayer environment that enables the membrane proteins to remainactive and stable. The concept of this technology is derived from high-densitylipoprotein (HDL) particles and apolipoprotein, their primary proteincomponent. The nano-disc is a non-covalent structure consisting of MSP that isgenetically engineered to mimic the activity of apolipoprotein A1 (Apo-A1) andphospholipid bilayer. In the presence of synthetic phospholipids, MSP1D1 canself-assemble into discoidal nanoparticlesknown as nano-discs. These nano-discs are suitable for studying membraneproteins and are applicable in the field of medicine and biotechnology.[1]
Legal Information
Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.
- 7,691,414 Membrane scaffold proteins
- 7,662,410 Membrane scaffold proteins and embedded membrane proteins
- 7,622,437 Tissue factor compositions and methods
- 7,592,008 Membrane scaffold proteins
- 7,575,763 Membrane scaffold proteins and tethered membrane proteins
- 7,083,958 Membrane scaffold proteins
- 7,048,949 Membrane scaffold proteins
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Cet article | |||
|---|---|---|---|
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli |
| assay ≥90% (SDS-GE) | assay ≥90% (SDS-GE) | assay ≥90% (SDS-GE) | assay ≥90% (SDS-GE) |
| mol wt 22,043.9 Da | mol wt - | mol wt - | mol wt - |
| form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| solubility water: soluble | solubility - | solubility - | solubility - |
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Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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