All critical reagents necessary to perform the assay are provided in the kit. The plate-based format avoids the high cost and potential LC column blockage associated with bead-based enrichment formats. Sample preparation with the SILu™MAb PK kit can be performed in under 5 hours and consists of coating wells with high-specificity capture antibody, followed by immunoaffinity enrichment and rapid, in-plate trypsin digestion. The resulting digest is ready for injection, with no SPE or other cleanup required.
Application
The SILu™MAb K1 Pharmacokinetic (PK) kit enables a robust, high-throughput assay for quantification of human IgG1 antibody in animal sera by LC-MS/MS.
Stockage et stabilité
Store the kit at 2-8°C. The kit is stable for two years refrigerated.
Rapid trypsin digest kit yields reliable results in less than 2 hours for mass spectrometry analysis.
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