MBD0032

Eubacteria FISH probe - ATTO488

Probe for fluorescence in situ hybridization (FISH), 20 µM in water

• Synonyme(s) : Fluorescent Probe
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.55

Propriétés

• Niveau de qualité: 200
• Technique(s): FISH: suitable
• Fluorescence: λ_ex 504 nm; λ_em 521 nm (ATTO488)
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

Fluorescent In Situ Hybridization technique (FISH) is based on the hybridization of fluorescent labeled oligonucleotide probe to a specific complementary DNA or RNA sequence in whole and intact cells.[1] Microbial FISH allows the visualization, identification and isolation of bacteria due to recognition of ribosomal RNA also in unculturable samples.[2]

FISH technique can serve as a powerful tool in the microbiome research field by allowing the observation of native microbial populations in diverse microbiome environments, such as samples from human origin (blood[3] and tissue[4]), microbial ecology (solid biofilms[5] and aquatic systems[6]) and plants[7].

Prokaryotic single cell life forms are divided into two domains, called Bacteria and Archaea, originally categorized as Eubacteria and Archaebacteria.[8] However both terms, Eubacteria and Bacteria are still being used in microbiology. Eubacteria probe recognizes most bacteria as it is complementary to a portion of 16S rRNA found in almost all bacteria.^[9],[10]
FISH technique was successfully used to identify different bacteria with the universal bacterial probe in various samples such as, pure culture (as described in the figure legends), blood cultures^[10],[11], periapical tooth lesions¹², saliva¹³, biofilms from voice prostheses¹⁴, subgingival biofilm¹⁵, aortic wall tissue¹⁶, buccal epithelial cells, pure culture and cell culture¹⁷, intestine tissue embedded in paraffin¹⁸, necrotizing fasciitis and pure culture¹⁹, colon sections embedded in paraffin^20,21, cancer tissues^22,23, environmental samples²⁴ and gut of the medicinal leech²⁵. The probe can also be used for combined technique of FISH and Flow cytometric analysis. ^9,26,27

It is strongly recommended to include positive and negative controls in FISH assays to ensure specific binding of the probe of interest and appropriate protocol conditions. We offer positive (MBD0032/33) and negative (MBD0034/35) control probes, that accompany the specific probe of interest.

Application

Eubacteria FISH probe - ATTO488 is suitable to use as a probe for fluorescence in situ hybridization (FISH) to recognize Eubacteria cells .

Caractéristiques et avantages

• Visualize, identify and isolate bacteria cells.
• Observe native bacteria cell populations in diverse microbiome environments.
• Specific, sensitive and robust identification of bacteria cells in mixed microorganism population.
• Specific, sensitive and robust identification even when bacteria are in low abundance in the sample.
• FISH can complete PCR based detection methods by avoiding contaminant bacteria detection.
• Provides information on bacteria morphology and allows to study biofilm architecture.
• Identify various bacteria in environmental and clinical samples such as, formalin-fixed paraffin-embedded (FFPE) samples, blood cultures, saliva and more.
• The ability to detect bacteria in its natural habitat is an essential tool for studying host-microbiome interaction.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable