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MAK446

Sigma-Aldrich

Acid Phosphatase Assay Kit

Sufficient for 100 colorimetric tests

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Niveau de qualité

100

Entrée

serum
plasma
tissue sample(s)
cell lysate

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

cancer, prostate tumor; gastrointestinal diseases

Température de stockage

−20°C

Catégories apparentées

Description générale

Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used.

Application

The Acid Phosphatase Assay Kit may be used for:
  • Cancer Research
  • Gastrointestinal Research
  • Metabolism Research

Caractéristiques et avantages

Efficient and Precise: Requires only 20 μL of sample with a 30-minute assay reaction to detect acid phosphatase levels across a broad range from 0.05 to 60 U/L, providing both convenience and sensitivity.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Adéquation

The kit is suitable for the determination of acid phosphatase in biological samples (e.g. plasma, serum, cell lysate, tissue samples.)

Principe

The non-radioactive, colorimetric Acid Phosphatase Assay Kit is based on the cleavage of p-nitrophenol from the synthetic substrate. p-Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at A405 nm after addition of the stop reagent is directly proportional to the enzyme activity.

Autres remarques

For additional information on our range of Biochemicals, please complete this form.

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Organes cibles

Nervous system,Kidney,Liver,Skin

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

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