Cholesterol is a sterol and lipid present in the cell membranes, and is transported in the bloodstream of all animals. It is used to form cell membranes and hormones, and plays important roles in cell signaling processes. Elevated levels (hypercholesterolemia) have been associated with cardiovascular diseases such as atherosclerosis; whereas, low levels (hypocholesterolemia) may be linked to depression, cancer and cerebral hemorrhage.
Application
The AF Cholesterol Assay Kit may be used for:
Cancer Research
Cardiovascular Research
Neurological Research
Caractéristiques et avantages
Efficient and Precise: Detect Cholesterol levels ranging from 0.1 to 10 mg/dL (colorimetric) and 0.02 to 2 mg/dL (fluorimetric) in a 30 minute room temperature reaction, providing both convenience and sensitivity.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Adéquation
The kit is suitable for the quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism in serum, plasma etc.
Principe
Simple, direct and automation-ready procedures for measuring cholesterol are very desirable. The AF Cholesterol Assay Kit uses a single working reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at A570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the total cholesterol concentration in the sample.
Autres remarques
For additional information on our range of Biochemicals, please complete this form.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
