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MAK094

Sigma-Aldrich

Protein Carbonyl Content Assay Kit

sufficient for 100 colorimetric tests

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric tests

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

cardiovascular diseases; neurological disorders; cancer; gastrointestinal diseases

Température de stockage

2-8°C

Description générale

Oxidative stress results when the effectiveness of antioxidant defenses is insufficient to deal with the production of reactive oxygen species (ROS). ROS can induce damage to DNA, lipids, and proteins. The oxidation of proteins results in the production of stable carbonyl groups, which can be used as a measure of oxidative injury.

Application

Protein carbonyl content assay kit has been used to determine protein carbonyl concentration.[1][2][3][4][5]

Adéquation

Suitable for quantifying carbonyls in protein samples

Principe

The Protein Carbonyl Content Assay Kit provides a simple and direct procedure for measuring carbonyl content in a variety of biological samples. Carbonyl content is determined by the derivatization of protein carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH) leading to the formation of stable dinitrophenyl (DNP) hydrazone adducts, which can be detected spectrophotometrically at 375 nm, proportional to the carbonyls present.

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Réf. du produit
Description
Tarif

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

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Certificats d'analyse (COA)

Lot/Batch Number
8M13K08300
8F16K08300
8B07K08300
7L05K08300
7E05K08300

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Consulter la Bibliothèque de documents

Effects of chilled-then-frozen storage (up to 52 weeks) on an indicator of protein oxidation and indices of protein degradation in lamb M. longissimus lumborum.
Coombs CE, et al.
Meat Science, 135, 134-141 (2018)
Benjamin W B Holman et al.
Meat science, 139, 171-178 (2018-02-11)
Different chilled (~0.1 °C for up to 5 weeks) then frozen storage (up to 12 months) combinations and two frozen storage holding temperatures (-12 °C and -18 °C) effects on beef M. longissimus lumborum (LL) protein structure degradation and a marker of protein oxidation were
Melissa M Miranda-Cruz et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 213, 19-26 (2018-07-25)
Hypoxia inducible factor-1 (HIF-1) is a transcriptional factor that induces genes involved in glucose metabolism. HIF-1 is formed by a regulatory α-subunit (HIF-1α) and a constitutive β-subunit (HIF-1β). The white spot syndrome virus (WSSV) induces a shift in glucose metabolism
UV?B radiation interacts with temperature to determine animal performance.
Ghanizadeh-Kazerouni E, et al.
Functional Ecology, 30(4), 584-595 (2016)
Mohammad Arif et al.
Toxicology and industrial health, 34(4), 213-218 (2018-03-10)
Lead exposure can damage cells directly by effecting DNA or indirectly by modifying proteins and enzymes. In Bangladesh, many working children are exposed to a very high level of lead during their early life due to their involvement with lead-oriented
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Questions

1–3 sur 3 questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 réponse

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 réponse

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Could you please provide information on the type of buffer to use for isolating proteins from insect tissue in a way that will preserve the carbonylation for the MAK094-1KT Protein Carbonyl Content Assay Kit? Additionally, do you anticipate any adverse effects from Decolor paint pens on the cuticle of the insect tissue sample?

    1 réponse

    1. It is recommended to homogenize cells using mechanical cell disruption in water only, as common buffer components can interfere with the protein carbonyl content of the sample. If the protein is very dilute, it can be concentrated using a 10 kDa spin filter. For the assay, it is advised to use 100 µL of sample containing approximately 0.5-2 mg protein and include a reagent background control by using 100 µL of dH2O alone. Regarding the impact of xylenes on this assay, it would need to be confirmed empirically.

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