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MAK064

Sigma-Aldrich

Kit d′analyse du lactate

sufficient for 100 colorimetric or fluorometric tests

Synonyme(s) :

Kit d’analyse de l’acide lactique

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric or fluorometric tests

Application(s)

cosmetics
food and beverages

Méthode de détection

colorimetric
fluorometric

Maladie(s) pertinente(s)

endocrinological disorders, diabetes; cancer

Température de stockage

−20°C

Description générale

Le L(+)-lactate est un composé métabolique formé chez l′animal par l′action d′une enzyme, la lactate déshydrogénase. Le lactate est produit par les cellules prolifératives et en conditions anaérobies telles que l′exercice physique. Des concentrations anormalement élevées de lactate ont été associées à des états pathologiques tels que le cancer, le diabète et l′acidose lactique. Le L(+)-lactate est le principal stéréo-isomère du lactate formé lors du métabolisme intermédiaire de l′homme et est présent dans le sang à un taux d′environ 1 à 2 mmol/l. Le kit est un outil pratique de détection du L(+)-lactate dans les échantillons biologiques. Aucun prétraitement ni aucune purification des échantillons ne sont nécessaires.

Application

Le Kit d′analyse du lactate est utilisé pour doser le lactate dans les échantillons.[1][2]

Adéquation

Permet de détecter le L(+)-lactate dans les milieux de culture, les milieux de fermentation, le sang et les cellules.

Principe

Dans cette analyse, la concentration en lactate est déterminée par un dosage enzymatique donnant un produit mesurable par colorimétrie (570 nm) / fluorimétrie (λex = 535 nm/λem = 587 nm), dont la quantité est proportionnelle à celle du lactate présent. Le niveau de sensibilité de ce kit est typiquement situé entre 0,001 et 10 mM.

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Réf. du produit
Description
Tarif

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H317,H334

Classification des risques

Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

188.6 °F - closed cup

Point d'éclair (°C)

87 °C - closed cup

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
8J15K06070
8A17K06070
8B23K06070
8L03K06070
8F30K06070

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Min Xu et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 47(1), 151-160 (2018-05-16)
Poor response to chemotherapy leads to the relapse and metastatic progression of tumors. Reprogrammed glucose metabolism is one of the important hallmarks of cancer that facilitates cancer cell survival, proliferation and chemoresistance. However, the precise fate of glucose metabolism and
Prabha Shrestha et al.
PLoS pathogens, 13(9), e1006628-e1006628 (2017-09-19)
Primary effusion lymphoma (PEL) is an aggressive B-cell lymphoma with poor prognosis caused by Kaposi's sarcoma-associated herpesvirus (KSHV). Previous studies have revealed that HIF-1α, which mediates much of the cellular response to hypoxia, plays an important role in life cycle
Bibi Uhre Nielsen et al.
PloS one, 15(9), e0238524-e0238524 (2020-09-12)
Markers of lung inflammation measured directly in expectorated sputum have the potential of improving the timing of antibiotic treatment in cystic fibrosis (CF). L-Lactate might be a marker of inflammation, as it is produced from glucose by polymorphonuclear neutrophils (PMNs)
Annasofia Anemone et al.
International journal of oncology, 51(2), 498-506 (2017-07-18)
Dichloroacetate (DCA) can reverse the glycolytic phenotype that is responsible of increased lactate production and extracellular pH acidification in cancer cells. Magnetic resonance imaging-chemical exchange saturation transfer (MRI-CEST) pH mapping is a novel non-invasive imaging approach that can measure in vivo
Cardiac STAT3 Deficiency Impairs Contractility and Metabolic Homeostasis in Hypertension.
Altara R, et al.
Frontiers in Pharmacology, 7, 436-436 (2016)
Afficher tous les articles scientifiques apparentés

Articles

Aerobic Glycolysis and the Warburg Effect

Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.

Questions

1–9 sur 9 questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 réponse

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 réponse

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. What is the formula for the MAK064D Lactate standard?

    1 réponse

    1. The standard provided in the MAK064-1KT Lactate Assay Kit is L-Lactic acid sodium salt (CAS 867-56-1) dissolved in water. It contains no other components or cofactors.

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  4. Since the L-(+)-Lactate standard is not a pure solution of sodium L-(+)-Lactate in water or buffer, which are the cofactors present in the standard?

    1 réponse

    1. The standard provided in this kit is L-Lactic acid sodium salt (CAS 867-56-1) dissolved in water. There are no other components or cofactors present in the standard.

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  5. What is the concentration range of lactate for this? The description says typical sensitivity is 0.001 - 10 mM, but in the product info the standard curve for colorimetric detection appears to go to a max. concentration of 0.0002µM (10nmol in 50µL sample)

    1 réponse

    1. The lower limit of detection is calculated by dividing the lowest concentration standard by the maximum sample volume. The higher limit of detection is determined by dividing the highest concentration standard by the minimum sample volume.

      For the higher limit of detection, take the 10 nmol standard and divide by 1 uL to get 10 mM. For the lower limit of detection, take 0.02 nmol and divide by 50 uL to get 0.0004 mM. We have reached out to our vendor to discuss the range calculation further, as it seems there is some conflicting information.

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  6. Can this kit be used on conditioned media containing phenol red?

    1 réponse

    1. If the sample being measured is the cell culture supernatant, the required volume is 20 uL. This is diluted with a total volume of 100 uL of the various kit reagents. The presence of phenol red may impact the results. It would be up to the end user to determine suitability.

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  7. On which enzymatic reaction is this kit based? Does pyruvate in the sample interfere with the assay?

    1 réponse

    1. This kit utilizes lactate oxidase to measure lactate. In sufficient amounts pyrivate may impact the results. This can be corrected by subtracting any background from the blank value. See page 3 in the kit protocol provided in the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/411/763/mak064bul.pdf

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  8. What is the Department of Transportation shipping information for this product?

    1 réponse

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  9. Can I make up my own Lactate standard to use with Product MAK064, Lactate Assay kit?

    1 réponse

    1. Unfortunately, no. If you run the 100 mM L-(+)-Lactate standard (MAK064D) that comes with the kit, you should get good results. However, if you just use, for example, a solution of sodium L-(+)-lactate dissolved in water or buffer, you probably won't get good results. There are cofactors present in biological samples and in the L-(+)-Lactate standard that comes with the kit (which is not a pure solution of sodium L-(+)-Lactate in water or buffer). If such a "homemade" standard solution were to be spiked with a biological sample or the kit standard, the calculations would be more complicated, but the assay should work.

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