sufficient for 100 colorimetric or fluorometric tests
Méthode de détection
colorimetric fluorometric
Maladie(s) pertinente(s)
neurological disorders; gastrointestinal diseases; cancer
Température de stockage
−20°C
Description générale
Phosphatidylcholine (PC) accounts for more than 50% of the phospholipids composing mammalian plasma membranes and is particularly enriched in the extracellular leaflet. PC is synthesized in the liver and is required for lipoprotein assembly and secretion. PC is cleaved by phospholipase D, which hydrolyses the choline head, producing the signaling intermediate phosphatidic acid.
Application
Phosphatidylcholine Assay Kit has been used to determine the concentration of phosphatidylcholine in samples.[1][2][3]
Adéquation
Suitable for the measurement of phosphatidylcholine in a variety of biological samples
Principe
In this assay, PC concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the PC present.
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Hepatocellular carcinoma (HCC) is the third most common cause of cancer-related death in the world. Choline deficiency has been well studied in the context of liver disease; however, less is known about the effects of choline supplementation in HCC. The
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
