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MAK038

Sigma-Aldrich

NADP/NADPH Quantitation Kit

sufficient for 100 colorimetric tests

Synonyme(s) :

NADP/NADPH Assay Kit

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About This Item

Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NA.25

Utilisation

sufficient for 100 colorimetric tests

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

cancer

Conditions d'expédition

dry ice

Température de stockage

−70°C

Description générale

Nicotinamide adenine dinucleotide phosphate (NADP) is an enzymatic cofactor involved in many redox reactions where it cycles between the reduced (NADPH) and oxidized (NADP) forms. NADP is also involved in biosynthetic reactions such as lipid and nucleic acid synthesis where it functions as a reducing agent. The oxidative branch of the Pentose Phosphate Pathway (PPP) is the major source of NADPH produced in animal cells.

Application

NADP/NADPH (nicotinamide adenine dinucleotide phosphate) Quantitation Kit has been used for quantification of NADP/NADPH. It has been used to quantify NAD(P)H in order to determine SHMT2 (serine hydroxymethyltransferase 2) specific enzyme activity.

Adéquation

Suitable for the detection of NADP, NADPH and their ratio in a variety of biological samples including cell and tissue lysates

Principe

The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides, NADP (nicotinamide adenine dinucleotide phosphate) and NADPH, and their ratio. There is no requirement to purify NADP/NADPH from samples. This assay is specific for NADP and NADPH and does not detect NAD or NADH. NADPt (NADP and NADPH) or NADPH are quantified in a colorimetric assay (450 nm).

Remplacé(e)(s) par

Réf. du produit
Description
Tarif

Pictogrammes

Health hazardCorrosion
GHS08,GHS05

Mention d'avertissement

Danger

Mentions de danger

H314,H317,H334

Classification des risques

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Cell death & disease, 9(5), 572-572 (2018-05-16)
Pentose phosphate pathway (PPP) is a major glucose metabolism pathway, which has a fundamental role in cancer growth and metastasis. Even though PPP blockade has been pointed out as a very promising strategy against cancer, effective anti-PPP agents are not
Q Shi et al.
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It has been well established that inflammation and concurrent mutagenic exposures drive the carcinogenic process in a synergistic way. To elucidate the role of the inflammatory cytokine IL-8 in this process, we studied its effect on the activation and deactivation
Mechanisms of action of isoniazid.
Timmins G S and Vojo D
Molecular Microbiology, 62(5), 1220-1227 (2006)
Andreas Carstensen et al.
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Phosphorus (P) is an essential macronutrient, and P deficiency limits plant productivity. Recent work showed that P deficiency affects electron transport to photosystem I (PSI), but the underlying mechanisms are unknown. Here, we present a comprehensive biological model describing how
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Metabolic engineering, 4(3), 217-229 (2002)
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