This antibody product is in mouse ascites fluid and has not been purified. The total IgG1 concentration is reported in the product Certificate of Analysis and will range from 3.0 - 8.0 mg/mL. The Quality testing included optimization of the dilution factor for usage in Western Blot. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/m5546#product-documentation
M5546
Anti-c-Myc Tag Antibody
mouse monoclonal, 9E10
Synonyme(s) :
Anti-c-Myc
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100 μL
560,00 €
0.2 ML
725,00 €
560,00 €
Disponible pour expédition le11 avril 2025Détails
Un anticorps recombinant, sans conservateur, est disponible pour votre cible. Essayez ZRB1003
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100 μL
560,00 €
0.2 ML
725,00 €
About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
560,00 €
Disponible pour expédition le11 avril 2025Détails
Un anticorps recombinant, sans conservateur, est disponible pour votre cible. Essayez ZRB1003
Devis pour commande en gros
Produits recommandés
Nom du produit
Anticorps anti-c-Myc, clone 9E10, ascites fluid
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
ascites fluid
Type de produit anticorps
primary antibodies
Clone
9E10, monoclonal
Contient
15 mM sodium azide
Espèces réactives
human
Technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:100 using c-myc tagged fusion protein
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... MYC(4609)
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Description générale
MYC (MYC proto-oncogene) is a transcriptional factor and oncoprotein. c-myc is a member of MYC gene family. It is located on human chromosome 8q24. c-Myc gene codes for basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor.
Monoclonal Anti-c-myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice.
Monoclonal Anti-c-myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice.
c-Myc is a transcription factor which is over-expressed in a wide range of cancers.
Spécificité
Recognizes both N- terminal and C-terminal c-Myc tagged proteins.
Immunogène
peptide synthétique de la protéine p62c-Myc humaine.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Monoclonal anti-c-Myc antibody produced in mouse has been used for Western blotting and immunohistochemistry.[1]
Monoclonal Anti-c-Myc antibody has also been used in:
Monoclonal Anti-c-Myc antibody has also been used in:
- the detection of horseradish peroxidase conjugate in phosphate buffered saline tween-20 (PBST) and 65-kDa mannoprotein (MP65) Dabs
- immunoblotting
- immunoprecipitation
- biochemical and histological studies
Actions biochimiques/physiologiques
The cellular myelocytomatosis (c-myc) oncogene plays a major role in cellular proliferation, differentiation and apoptosis. It acts as a transcriptional regulator of gene expression. MYC (MYC proto-oncogene) may be essential for cancer initiation, promotion and therapy resistance. Overexpression of MYC in DLBCL (diffuse large B-cell lymphoma) results in the poor outcome and invasive treatment, when medicated with rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone (R-CHOP).
c-Myc is a regulator of the cell cycle and aids in the growth of tumors in the liver. Translocations in the gene encoding it have been shown to be linked to Burkitt lymphoma. It has been studied that c-Myc targets many protein-coding genes which enhances the oncogenic properties of c-Myc.
Forme physique
The product is provided as ascites fluid with 15 mM sodium azide as a preservative.
Stockage et stabilité
For continuous use, store at 2-8 °C for a maximum of one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Certificats d'analyse (COA)
Lot/Batch Number
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Les clients ont également consulté
A PTK7/Ror2 Co-receptor complex affects xenopus neural crest migration.
Podleschny M, et al.
PLoS ONE, 10(12), e0145169-e0145169 (2015)
MYC Protein?positive Diffuse Large B-Cell Lymphoma Features an Activated B-Cell Receptor Signal Pathway.
Wang WG, et al.
American Journal of Surgical Pathology, 41(4), 541-549 (2017)
Structure-function analysis of Nel, a thrombospondin-1-like glycoprotein involved in neural development and functions.
Nakamura R, et al.
The Journal of Biological Chemistry, 287(5), 3282-3291 (2012)
Clinicopathological significance of c-MYC in esophageal squamous cell carcinoma.
Lian Y, et al.
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine, 39(7), 1010428317715804-1010428317715804 (2017)
8q24 prostate, breast, and colon cancer risk loci show tissue-specific long-range interaction with MYC.
Ahmadiyeh N, et al.
Proceedings of the National Academy of Sciences of the USA, 107(21), 9742-9746 (2010)
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