M5293
Monoclonal Anti-Mucin Gastric antibody produced in mouse
clone 45M1, ascites fluid
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About This Item
Produits recommandés
Source biologique
mouse
Conjugué
unconjugated
Forme d'anticorps
ascites fluid
Type de produit anticorps
primary antibodies
Clone
45M1, monoclonal
Contient
15 mM sodium azide
Espèces réactives
chicken, monkey, rat, rabbit, pig, hedgehog, mouse, human, feline
Technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using sections of human stomach
western blot: suitable using non-reducing conditions
Isotype
IgG1
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... MUC5AC(4586)
mouse ... Muc5ac(17833)
rat ... Muc5ac(682837)
Description générale
Monoclonal Anti-Human Gastric Mucin (mouse IgG1 isotype) is derived from the 445M1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with mucin isolated from human ovarian cyst fluid. Mucin is a high M.W. (1,000 kDa) glycoprotein, expressed by mucus cells of the gastric epithelium and by goblet cells of the fetal, precancerous and cancerous colon, but not by those of the normal colon. It also appears in other epithelial tissues, which are embryologically derived from the foregut (epigastric and bronchial epithelium) and in Müller ducts (mucus cells of the endocervix and urethral epithelium near the prostatic utriculus).
Mucin has a netlike, branched structure and is a secreted protein. It belongs to the category of gel-forming mucins. The gene encoding this protein is upregulated by interleukin-1β (IL-1β) and is localized on chromosome 11p15.5.
Spécificité
The antibody recognizes the mucin epitope g located in the peptide core of gastric mucin (>1,000 kDa). This epitope is completely destroyed by thiol reduction (using mercaptoethanol) and partially lost following trypsin proteolysis, but is stable upon periodate oxidation. The antibody stains the surface gastric epithelium of normal human gastrointestinal tract and reacts with fetal, pre-cancerous and cancerous colonic mucosa, but not with normal colon.
Immunogène
mucin from human ovarian cyst fluid.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
Monoclonal Anti-Human Gastric Mucin may be used for the localization of gastric mucin using various immunochemical assays such as immunoblot, immunofluorescence, immunohistochemistry, immunostaining and immunoradiofixation.
Actions biochimiques/physiologiques
Mucin is composed of a peptide core containing heavily glycosylated regions and nonglycosylated regions. The heavily glycosylated components of mucin consist of a mosaic of epitopes, some of which are associated with saccharide moieties and are related to blood-group antigens, and others which are M1 antigens associated with the peptidic core. M1 antigens are common to human ovarian mucinous cyst fluids and to gastric mucosa. M1 is distributed in various human tumors such as the colon, stomach, pancreas, ovary, Barrets′ esophagus, endocervix, and endometrium carcinomas.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Les clients ont également consulté
Emma C Skoog et al.
PloS one, 7(5), e36378-e36378 (2012-05-09)
Helicobacter pylori colonizes the mucus niche of the gastric mucosa and is a risk factor for gastritis, ulcers and cancer. The main components of the mucus layer are heavily glycosylated mucins, to which H. pylori can adhere. Mucin glycosylation differs
Optimized Protocol for Regeneration of the Conjunctival Epithelium Using the Cell Suspension Technique.
Bertolin M, et al.
Cornea, 38(4), 469-479 (2019)
Abnormal expression of M1/MUC5AC mucin in distal colon of patients with diverticulitis, ulcerative colitis and cancer.
Forgue-Lafitte ME, et al.
International Journal of Cancer. Journal International Du Cancer, 121(7), 1543-1549 (2007)
Blocking peripheral serotonin synthesis by telotristat etiprate (LX1032/LX1606) reduces severity of both chemical-and infection-induced intestinal inflammation.
Kim JJ, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology, 309(6), G455-G465 (2015)
Gastrointestinal cell lines form polarized epithelia with an adherent mucus layer when cultured in semi-wet interfaces with mechanical stimulation.
Navabi N, et al.
PLoS ONE, 8(7), e68761-e68761 (2013)
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