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M3534

Sigma-Aldrich

Anti-Mouse IgG (Fc specific) antibody produced in goat

2.0 mg/mL, affinity isolated antibody

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

goat

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Concentration

2.0 mg/mL

Technique(s)

indirect ELISA: 1:15,000

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

IgG subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids.

Immunogène

Mouse IgG, Fc fragment

Application

Anti-Mouse IgG (Fc specific) antibody has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • Ouchterlony double diffusion
  • anti-drug antibody immunoassay
  • immunoelectrophoresis

Actions biochimiques/physiologiques

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice

Autres remarques

Antibody adsorbed with human IgG and rat serum proteins

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Notes préparatoires

Adsorbed to reduce background with human or rat samples.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Nathan H Joh et al.
Journal of pharmaceutical sciences, 109(1), 845-853 (2019-10-20)
Silicone oil is a lubricant for prefilled syringes (PFS), a common primary container for biotherapeutics. Silicone oil particles (SiOP) shed from PFS are a concern for patients due to their potential for increased immunogenicity and therefore also of regulatory concern.
Integrating molecular detection and response to create self-signalling antibodies
Randle B, et al.
Biochemical and Biophysical Research Communications, 324(2), 504-510 (2004)
Universal immunoassay applied during early development of large molecules to understand impact of immunogenicity on biotherapeutic exposure
Bautista AC, et al.
The APPS Journal, 14(4), 843-849 (2012)
Christiano M V Barbosa et al.
Cell death discovery, 5, 135-135 (2019-09-26)
Annexin A1 (AnxA1) modulates neutrophil life span and bone marrow/blood cell trafficking thorough activation of formyl-peptide receptors (FPRs). Here, we investigated the effect of exogenous AnxA1 on haematopoiesis in the mouse. Treatment of C57BL/6 mice with recombinant AnxA1 (rAnxA1) reduced
Development and validation of a standardized ELISA for the detection of soluble Fc-epsilon-RI in human serum
Lexmond W, et al.
Journal of Immunological Methods, 373(1-2), 192-199 (2011)

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