M062000
Amino-ON CPG
pore size 500 Å, 30-40 μmol/g
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| À vous/Référence | Disponibilité | Prix |
|---|---|---|
0.25 g | Vérifier la disponibilité des articles du panier | 134,00 € |
1 g | Vérifier la disponibilité des articles du panier | 501,00 € |
A propos de cet article
NACRES:
NA.51
UNSPSC Code:
12352200
Service technique
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type
for DNA synthesis
product line
Proligo Reagents
pore size
500 Å
λ
conforms (UV/VIS Identity)
storage temp.
−20°C
General description
Amino-ON CPG can be employed to introduce a covalently bound, primary amino group at the 3′-end of an oligonucleotide. It comprises a protected hexylamino group. The free amine is liberated under the alkaline conditions that are employed to cleave and deprotect the oligonucleotide on the support, while the hexylamino group remains attached to the oligonucleotide. The Amino-ON CPG further comprises a DMT-protective group which is removed in the first synthesis cycle. The synthesis of oligonucleotides is conducted in the same manner as with nucleoside-loaded CPG, without changes in any of the reagents of the synthesis. Amino-ON CPG can be employed to conjugate biotin, fluorescein or other modifiers and reporter groups to the 3′-end of oligonucleotides or to attach oligonucleotides to surfaces. Proligo′s Amino-ON CPG combines the advanced features of cleavage and deprotection under mild conditions, and maintenance of the integrity of the 3′-modification with high synthesis yields.
Features and Benefits
- Introduces a primary amino group attached to a C6-linker
- Fully compatible with standard phosphoramidite reagents and synthesis conditions
- Can be used with DNA and RNA; 5′-oligonucleotide modifications such as fluorescein or biotin; and with the synthesis of thioated DNA oligonucleotides
- Compatible with dG(dmf) fast deprotection chemistry: the oligonucleotide can be cleaved from the support and deprotected in concentrated ammonia at 55 °C in 2 hours
- Provides all the advantages of homogeneous pore-size porous glass supports
- DMT-protected in the same way as standard nucleoside-loaded CPG
Legal Information
This product and it use is covered under US Patent Number 7,329,515
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Cet article | ||
|---|---|---|
| description pore size 500 Å, 30-40 μmol/g | description pore size 1000 Å, 25-35 μmol/g | description pore size 500 Å, 30-40 μmol/g |
| Quality Level 200 | Quality Level 200 | Quality Level 200 |
| product line Proligo Reagents | product line Proligo Reagents | product line Proligo Reagents |
| type for DNA synthesis | type - | type - |
| pore size 500 Å | pore size 1000 Å | pore size 500 Å |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| λ conforms (UV/VIS Identity) | λ - | λ - |
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Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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