L7914
Lipoprotein, low density from human plasma
≥95% (SDS-PAGE), solution
Synonyme(s) :
β-Lipoprotein, LDL, Low density lipoprotein
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About This Item
Produits recommandés
Source biologique
human plasma
Niveau de qualité
Pureté
≥95% (SDS-PAGE)
Forme
solution
Groupe fonctionnel
ester
phospholipid
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Informations sur le gène
human ... APOB(338) , APOC2(344) , APOE(348)
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Application
Lipoprotein, low density from human plasma has been used:
- as an additive in cholesterol-free RPMI media
- as a component in buffer to perform an assay for measuring lipid peroxidation
- to stimulate hepatic macrophages
- as a plasma protein to determine pazopanib unbound fraction (fu%) by equilibrium dialysis
Actions biochimiques/physiologiques
LDL and HDL transport both dietary and endogenous cholesterol in the plasma. LDL is the main transporter of cholesterol and cholesteryl esters and makes up more than half of the total lipoprotein in plasma. LDL is absorbed by the liver and other tissues via receptor mediated endocytosis. The cytoplasmic domain of the LDL receptor facilitates the formation of coated pits; receptor-rich regions of the membrane. The ligand binding domain of the receptor recognizes apo-B100 on LDL, resulting in the formation of a clathrin-coated vesicle. ATP-dependent proton pumps lower the pH inside the vesicle resulting dissociation of LDL from its receptor. After loss of the clathrin coat the vesicles fuse with lysozomes, resulting in peptide and cholesteryl ester enzymatic hydrolysis. The LDL receptor can be recycled to the cell membrane. Insulin, tri-iodothyronine and dexamethasome have shown to be involved with the regulation of LDL receptor mediated uptake.
Attention
Freezing of lipoprotein solutions may cause structural or compositional changes.
Propriétés physiques
Low density lipoproteins are smaller than VLDL and IDL (26 nm) (MW approximately 3.5 million) and more dense (~1.04). The protein component of LDL is apolipoprotein B100. LDL contains 20-22% protein, 10-15% triglycerides, 20-28% phospholipids, 37-48% cholesteryl esters and 8-10% cholesterol.
Forme physique
Solution in 150 mM NaCl and 0.01% EDTA, pH 7.4
Autres remarques
View more information on lipoprotein function and lipid transport at www.sigma-aldrich.com/enzymeexplorer
Clause de non-responsabilité
Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du ministère français de la recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Les clients ont également consulté
Leanne Satchell et al.
Biochemistry, 51(18), 3767-3775 (2012-04-13)
Low-density lipoprotein (LDL) has recently been shown to be oxidized by iron within the lysosomes of macrophages, and this is a novel potential mechanism for LDL oxidation in atherosclerosis. Our aim was to characterize the chemical and physical changes induced
L L Rudel et al.
The Biochemical journal, 139(1), 89-95 (1974-04-01)
1. A simple method for isolation of individual human plasma lipoprotein classes is presented. In this technique, lipoproteins are removed from plasma at d1.225 by ultracentrifugation, after which they are separated and purified by agarose-column chromatography. 2. Three major classes
S P Zhao et al.
Clinica chimica acta; international journal of clinical chemistry, 203(2-3), 109-117 (1991-12-16)
After 15 weeks of simvastatin therapy (20 mg/day), low density lipoprotein particle size in sera of 16 patients with type IIb hyperlipoproteinemia increased significantly from 233 +/- 5.0 A to 237 +/- 7.0 A (P less than 0.05), analyzed by
Determination of unbound fraction of pazopanib in vitro and in cancer patients reveals albumin as the main binding site
Imbs DC, et al.
Investigational New Drugs, 34(1), 41-48 (2016)
Matthew R Sobansky et al.
Analytical and bioanalytical chemistry, 403(2), 563-571 (2012-02-23)
Columns containing immobilized low-density lipoprotein (LDL) were prepared for the analysis of drug interactions with this agent by high-performance affinity chromatography (HPAC). R/S-Propranolol was used as a model drug for this study. The LDL columns gave reproducible binding to propranolol
Articles
Lipoproteins package cholesterol for transport in plasma, essential for lipid transport and cellular function in the body.
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