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L6669

Sigma-Aldrich

LuminoCt® qPCR ReadyMix™

For fast probe-based quantitative PCR

Synonyme(s) :

LuminoCt® qPCR ReadyMix™, LuminoCt®

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About This Item

Code UNSPSC :
41106300
Nomenclature NACRES :
NA.55

Forme

liquid

Utilisation

sufficient for 100 reactions
 sufficient for 2000 reactions
 sufficient for 500 reactions

Caractéristiques

dNTPs included
hotstart

Technique(s)

qPCR: suitable

Couleur

colorless

Entrée

purified DNA

Adéquation

suitable for (quantitative PCR)

Application(s)

agriculture

Compatibilité

Bio-Rad MyiQ ( )
 Bio-Rad iCycler iQ
 Bio-Rad iQ 5
for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000

Méthode de détection

probe-based

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

Inclusion of JumpStart™ Taq antibody in the ReadyMix™ eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup.

  • Kit is designed to perform probe-based qPCR assays on commonly available real-time instrument platforms.
  • ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays.
  • Kit is not compatible with qPCR instruments that utilize glass capillary tubes.
LuminoCt® qPCR ReadyMix combines the performance enhancements of our JumpStart™ Taq antibody for hot start PCR with the convenience of an easy-to-use reaction mixture.

Application

LuminoCt® qPCR ReadyMix™ has been used in 2-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay to quantify the reverse transcription product or cDNA for gene expression analysis. It has also been used in qPCR for quantifying the RNA extracted from rhinoviruses.

Caractéristiques et avantages

  • Ideal for rapid, high-throughput 2-step qPCR protocols
  • The master mix allows consistency and reproducibility from one reaction to the next
  • Reduced preparation time and the risk of contamination from multiple pipetting steps
  • Compatible with commonly available real-time instrument platforms for probe-based qPCR assays
  • Require small amplicons (<200 bp) for optimal results
  • Compatible with commercial primer sets, including TaqMan® assays
  • JumpStart™ Taq DNA polymerase allows room temperature set-up and the hot-start mechanism prevents primer-dimer and non-specific product formation
  • Assay results in as little as 25 minutes
  • Designed to deliver unsurpassed accuracy, precision, and sensitivity
  • Extensive design and validation of ReadyMix chemistry virtually eliminates the need to optimize assay parameters

Conditionnement

LuminoCt qPCR ReadyMix, Catalog Number L5794, contains optimized concentrations of Tris-HCl, pH 8.3, KCl, dNTPs (dATP, dCTP, dGTP, TTP), stabilizers, MgCl2 and JumpStart Taq DNA Polymerase. Provided as 100, 500 and 2000 reactions (25 μL mix in a 50 μL reaction volume).

100x ROX internal reference dye, Catalog Number R4526. Optional, for use with machines compatible with an internal reference dye, e.g., ABI and Stratagene.

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL

Autres remarques

  • Commercially available 5′ nuclease assays (e.g., TaqMan® Assays) are compatible with LuminoCt ReadyMix.
  • At a minimum, use software to design primers and probes for assays and ensure that qPCR amplicons are <200 bp.
  • Sigma recommends designing primers and probes with Beacon Designer software

To order primers and probes from Sigma, visit www.sigma.com/oligos

Informations légales

Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
LuminoCt is a registered trademark of Merck KGaA, Darmstadt, Germany
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • R4526100X ROX internal reference dyeFDS

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

W1754

Eau, PCR Reagent

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Juan Vicente Canet et al.
The Plant cell, 24(10), 4220-4235 (2012-10-16)
Salicylic acid (SA) signaling acts in defense and plant development. The only gene demonstrated to be required for the response to SA is Arabidopsis thaliana non-expresser of pathogenesis-related gene 1 (NPR1), and npr1 mutants are insensitive to SA. By focusing
Talita B Gagliardi et al.
PLoS pathogens, 18(1), e1010159-e1010159 (2022-01-08)
The clinical impact of rhinovirus C (RV-C) is well-documented; yet, the viral life cycle remains poorly defined. Thus, we characterized RV-C15 replication at the single-cell level and its impact on the human airway epithelium (HAE) using a physiologically-relevant in vitro
Samer M Hussein et al.
Nature, 471(7336), 58-62 (2011-03-04)
The mechanisms underlying the low efficiency of reprogramming somatic cells into induced pluripotent stem (iPS) cells are poorly understood. There is a clear need to study whether the reprogramming process itself compromises genomic integrity and, through this, the efficiency of
Laura de Diego-García et al.
Biochimica et biophysica acta. Molecular basis of disease, 1863(1), 43-51 (2016-10-22)
The Ubiquitin-Proteasome System (UPS) is essential for the regulation of the cellular proteostasis. Indeed, it has been postulated that an UPS dysregulation is the common mechanism that underlies several neurological disorders. Considering that extracellular nucleotides, through their selective P2Y2 receptor
Philip Owens et al.
PloS one, 8(6), e67533-e67533 (2013-07-11)
Bone Morphogenetic Proteins (BMPs) are secreted cytokines that are part of the Transforming Growth Factor β (TGFβ) superfamily. BMPs have been shown to be highly expressed in human breast cancers, and loss of BMP signaling in mammary carcinomas has been
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Probe-based QPCR offers high sensitivity and specificity with fluorescent-labeled probes and various dye options for multiplex amplification.

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PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

Polymerase Chain Reaction

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La réaction en chaîne par polymérase (PCR) est l'une des techniques les plus couramment utilisées en biologie moléculaire. Le procédé de PCR implique trois étapes principales : la dénaturation, l'hybridation des amorces et l'élongation

Protocoles

Multiplex qPCR Protocol

Standard qPCR protocol with up to four detection probes at specified concentrations, simplifying reaction setup with LuminoCt ReadyMix.

qPCR with Single Detection Probe

qPCR protocol template with specific detection probes aids in single-target detection using LuminoCt® ReadyMix™.

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

SPUD Assay for Detection of Assay Inhibitors Protocol

SPUD assay identifies inhibitors in RNA or DNA samples, useful for analyzing numerous or low-copy targets.

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