Toutes les photos(1)

Principaux documents

COO/COA

Voir toute la documentation

J2128

Anti-phospho-c-Jun (pSer⁶³) antibody produced in rabbit

Name: Anti-phospho-c-Jun (pSer<SUP>63</SUP>) antibody produced in rabbit
Brand: SIGMA

affinity isolated antibody, buffered aqueous glycerol solution

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme

Toutes les photos(1)

About This Item

Numéro MDL:

MFCD01092289

Code UNSPSC :

12352203

Nomenclature NACRES :

NA.41

Source biologique

rabbit

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous glycerol solution

Poids mol.

antigen 48 kDa

Espèces réactives

rat, mouse, human

Technique(s)

flow cytometry: 1:200
microarray: suitable
western blot (chemiluminescent): 1:1,000 using extract of NIH3T3 anisomycin or UV-treated cells

Numéro d'accès UniProt

P05412

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

phosphorylation (pSer63)

Informations sur le gène

human ... JUN(3725)
mouse ... Jun(16476)

Spécificité

The antibody does not react with the corresponding phosphorylated form of JunD or JunB.

Immunogène

synthetic phosphoserine⁶³ peptide corresponding to residues around Ser⁶³ of human c-Jun, conjugated to KLH.

Application

Anti-phospho-c-Jun (pSer63) antibody produced in rabbit has been used in microarray analysis to identify the changes in the level of cellular proteins in cells inoculated with Equine influenza virus.

Actions biochimiques/physiologiques

The protooncogene c-Jun encodes a member of the jun family that forms a component of the transcription factor AP-1 (Activator Protein-1). It dimerizes with a member of the Fos family via the leucine-zipper motif to form AP-1, a factor involved in binding to TPA (12-O-tetradecanoylphorbol 13-acetate) response element (TRE) of various genes that include human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. The dimers formed are involved in the activation of AP-1 dependent genes. AP-1 regulates the transcription of mammalian DDI (DNA-damage-inducible) genes.

Forme physique

Solution in 10 mM HEPES sodium, pH 7.5, containing 150 mM sodium chloride, 100 μg/mL bovine serum albumin, and 50% glycerol.

Vous ne trouvez pas le bon produit ?

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Prolonged activation of jun and collagenase genes by tumour necrosis factor-alpha.

D A Brenner et al.

Nature, 337(6208), 661-663 (1989-02-16)

Tumour necrosis factor-alpha (TNF-alpha) is secreted by macrophages in response to inflammation, infection and cancer. Sublethal doses of recombinant TNF-alpha to rats causes cachexia, anaemia and inflammation. TNF-alpha plays a major part in tissue inflammation and remodelling by stimulating production

jun-D: a third member of the jun gene family.

K Ryder et al.

Proceedings of the National Academy of Sciences of the United States of America, 86(5), 1500-1503 (1989-03-01)

The protooncogene c-jun encodes a component of the transcription factor AP-1. Both murine c-jun and a related gene (jun-B) are rapidly activated in BALB/c3T3 cells by serum growth factors. We report here the cloning and analysis of a cDNA encoding

Rapid and preferential activation of the c-jun gene during the mammalian UV response.

Y Devary et al.

Molecular and cellular biology, 11(5), 2804-2811 (1991-05-01)

Exposure of mammalian cells to DNA-damaging agents leads to activation of a genetic response known as the UV response. Because several previously identified UV-inducible genes contain AP-1 binding sites within their promoters, we investigated the induction of AP-1 activity by

The jun proto-oncogene is positively autoregulated by its product, Jun/AP-1.

P Angel et al.

Cell, 55(5), 875-885 (1988-12-02)

Binding of the human transcription factor Jun/AP-1 to a conserved 8 bp nucleotide sequence (TRE) is responsible for increased transcription of different cellular genes in response to tumor promoters, such as TPA, and serum factors. Enhanced Jun/AP-1 activity in TPA-stimulated

The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation.