I6510
Monoclonal Anti-Human IgE antibody produced in mouse
clone GE-1, ascites fluid
Synonyme(s) :
Monoclonal Anti-Human IgE
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
ascites fluid
Type de produit anticorps
secondary antibodies
Clone
GE-1, monoclonal
Contient
15 mM sodium azide
Technique(s)
indirect ELISA: 1:5,000
Isotype
IgG2b
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
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Description générale
Binds human IgE; does not bind human IgG, IgA or IgM.
Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin E (IgE) antibodies are tetramers with two light chains (κ or λ) and two ε-heavy connected by numerous intrachain disulfide bonds. IgE exists in two isoforms, one is associated with the B cell receptor and the other being secreted by plasma cell. It has a short life span in plasma when compared to receptor bounded IgE.
Spécificité
Monoclonal Anti-Human IgE is immunospecific for human IgE. No cross-reactivity with human IgG, IgM or purified light chains is observed.
Application
To confirm that a chimaeric monoclonal antibody had episolon chain, an elisa was performed using monoclonal anti-human IgE antibody (clone GE1) at a 1:1000 dilution.
Actions biochimiques/physiologiques
Immunoglobulin E (IgE) is implicated in acute allergic reactions and chronic inflammatory allergic diseases, such as allergic asthma and chronic urticaria (CU). Receptor bounded IgE aids antigen uptake and modulates the differentiation and proliferation of B cells. It confers immunity against helminthic parasites. IgE is also involved in facilitating type I hypersensitivity reactions. It regulates mast cell homeostasis and also exhibits immunomodulatory effects.
Forme physique
The product is provided as ascites fluid with 0.1% sodium azide as a preservative.
Stockage et stabilité
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Remarque sur l'analyse
The antibody is tested for reactivity against myeloma IgE and allergy-elevated IgE.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Les clients ont également consulté
Hans C Oettgen
The Journal of allergy and clinical immunology, 137(6), 1631-1645 (2016-06-07)
Fifty years ago, after a long search, IgE emerged as the circulating factor responsible for triggering allergic reactions. Its extremely low concentration in plasma created significant hurdles for scientists working to reveal its identity. We now know that IgE levels
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Scientific reports, 12(1), 18924-18924 (2022-11-08)
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Marjoke M Verweij et al.
Cytometry. Part B, Clinical cytometry, 78(5), 302-307 (2010-06-10)
P38 mitogen-activated protein kinase (MAPK) is known to govern IgE-mediated basophil activation. Intracellular phosphorylated p38 MAPK (Pp38 MAPK) in IgE-activated basophils can be quantified flow cytometrically. To study whether Pp38 MAPK constitutes a potential novel read-out for flow-assisted diagnosis of
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