This product is sufficient for ≤10 assays. When reconstituted with 10 ml deionized water, each vial will yield a solution with a pH of approximately 7.3, containing the following components: 0.3 mM 4-Hydroxyazobenzene-2-carboxylic acid (HABA), 0.45 mg/ml avidin, 0.3 M sodium chloride (NaCl), 0.01 M N-[2-Hydroxyethyl]piperazine-NN-[2-ethanesulfonic acid] (HEPES), a buffer with a pKa of 7.5, 0.01 M magnesium chloride (MgCl2), and 0.02% sodium azide as a preservative.
H2153
HABA/Avidin Reagent
lyophilized powder
Synonyme(s) :
HABA Reagent
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1 VIAL
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46
Produits recommandés
Forme
lyophilized powder
Niveau de qualité
Utilisation
sufficient for ≤10 assays
Couleur
orange
Adéquation
suitable for spectrophotometric determination of biotin and biotin conjugates in a wide variety of samples
Température de stockage
2-8°C
Description générale
4-Hydroxyazobenzene-2-carboxylic acid (HABA) is an avidin-binding dye that can be stoichiometrically displaced by biotin. The subsequent change in absorption at A500 provides a basis for spectrophotometric determination of biotin levels in biological samples.
HABA/Avidin Reagent is specific for chromogenic applications that depend upon quantification of biotin.
Application
HABA/Avidin Reagent is suitable for spectrophotometric determination of biotin and biotin conjugates in a wide variety of samples.
Reagent is capable of detecting biotin at μMolar concentrations (i.e., 1 nmole per ml). Typically used to quantitate biotin in biotinylated compounds.
Reconstitution
Prepares a ready-to-use HABA/avidin solution in buffered saline, pH approx. 7.3, with preservative when reconstituted with 10 ml deionized water.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3
Code de la classe de stockage
13 - Non Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Les clients ont également consulté
A Einspanier et al.
Biology of reproduction, 61(2), 512-520 (1999-07-20)
Relaxin is a peptide hormone with a broad range of biological activities, related not only to parturition and lactation but possibly also to decidualization, implantation, and early pregnancy. The present study was designed to investigate the secretion pattern of relaxin
R S Niedbala et al.
Journal of biochemical and biophysical methods, 13(4-5), 205-210 (1986-11-01)
Parameters and conditions of an enzyme based assay for biotin and avidin are presented. Biotinylated glucose-6-phosphate dehydrogenase when complexed with avidin becomes inactivated. Thus it was possible to construct a competitive assay system for biotin. The assay is sensitive between
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I would like to know how many assays can be done with a VIAL KIT and how much material it contains,
1 réponse-
Utile ?
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how much HABA is in the vial
1 réponse-
When reconstituted to 10mL, the vial will contain 0.3 mM HABA. Based on the molecular weight of HABA, 242.23 gmol, the calculated mass per vial is 726.69 ug. Please see the link below to review additional informaition available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/378/451/h2153dat.pdfUtile ?
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