This product is soluble in water up to 20 mg/mL. Alternatively, a buffer such as phosphate-buffered saline at a neutral pH can be used. Stability in solution has not been tested.
H0267
Hemoglobin A0, Ferrous Stabilized human
lyophilized powder
Synonyme(s) :
Hemoglobin A0 Ferrous Stabilized, Hemoglobin-A0
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About This Item
Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352202
eCl@ss :
42030116
Nomenclature NACRES :
NA.61
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Source biologique
human
Niveau de qualité
Essai
97-100% (agarose gel electrophoresis)
Forme
lyophilized powder
Technique(s)
immunofluorescence: suitable
Solubilité
H2O: soluble 20 mg/mL
Adéquation
suitable for electrophoresis and chromatography standard
Numéro d'accès UniProt
Température de stockage
−20°C
Informations sur le gène
human ... HBB(3043)
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Description générale
Hemoglobin is the major component of red blood cells, and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is the most important respiratory protein of vertebrates by virtue of its ability to transport oxygen from the lungs to body tissues, and to facilitate the return transport of carbon dioxide.
has not been tested for functional equivalence against native preparations (unlyophilized ferrous hemoglobins).
Application
Hemoglobin A0, ferrous stabilized human has been used:
- as the non-fluorescent absorber in the preparation of tissue-mimicking phantoms[1]
- to see how coronary intraplaque hemorrhage evokes a novel atheroprotective macrophage phenotype[2]
- to study the interference of hemoglobin (Hb) A0, HbA2, and HbS in the assay of HbF[3]
Actions biochimiques/physiologiques
Variation in the haemoglobin (Hb) encoding genes results in haemoglobinopathies such as thalassaemia and atypical structural variants like Hb Lepore and HbE.[5]
Conditionnement
Package size indicates the amount of hemoglobin as determined by the procedure of Drabkin, D.L., J. Biol. Chem., 164, 703 (1946).
Reconstitution
When reconstituted with buffer, gives >85% ferrous hemoglobin.
Clause de non-responsabilité
Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du ministère français de la recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Les clients ont également consulté
Motasam Majedy et al.
Journal of biomedical optics, 27(7) (2021-12-02)
For optical methods to accurately assess hemoglobin oxygen saturation in vivo, an independently verifiable tissue-like standard is required for validation. For this purpose, we propose three hemoglobin preparations and evaluate methods to characterize them. To spectrally characterize three different hemoglobin
Seung Yup Lee et al.
Science advances, 6(47) (2020-11-22)
Pancreatic cancer is one of the deadliest cancers, with a 5-year survival rate of <10%. The current approach to confirming a tissue diagnosis, endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA), requires a time-consuming, qualitative cytology analysis and may be limited because of
Randi E Gislefoss et al.
Biopreservation and biobanking, 19(6), 525-530 (2021-10-07)
Background: During sampling and processing, blood samples can be affected by hemolysis. Information is lacking regarding hemolysis for biobank samples. There is a need for a method that can easily measure hemoglobin as an indicator of hemolysis in stored samples
Screening and diagnosis for haemoglobin disorders
Prevention of Thalassaemias and Other Haemoglobin Disorders, 1 (2013)
Vivide Tuan-Chyan Chang et al.
Neoplasia (New York, N.Y.), 11(4), 325-332 (2009-03-25)
Cervical cancer is the second most common female cancer worldwide. The ability to quantify physiological and morphological changes in the cervix is not only useful in the diagnosis of cervical precancers but also important in aiding the design of cost-effective
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Which the buffer solution can dissolve it, how to prepare the buffer solution? After dissolving, how to store and what is the stability?
1 réponse-
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From which buffer was Product H0267 lyophilized? Did this buffer contain primary amines?
1 réponse-
Before lyophilyzation, ficoll & sucrose are added stabilizers & the hemoglobin is dialyzed against 25 mM ammonium bicarbonate containing 0.1 mM EDTA. It is expected that after lyophilization, there should be virtually no or substantially reduced ammonium bicarbonate. Stabilizers are the major components of the final product.
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Does the product, H0267, contain HbA1c?
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The glycation level of this product, i.e., HbA1c, has not been characterized.
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What is the solution stability of Product H0267, Hemoglobin A0?
1 réponse-
Solutions of ferrous hemoglobin are not stable indefinitely due to the likely autoxidation of the heme group leading to the formation of the ferric derivative (methemoglobin). It is recommended to prepare solutions fresh in deaerated water or buffer. If necessary, store such prepared solutions no more than 1-2 days at 2-8°C.
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Is there methemoglobin in Product H0267, Hemoglobin A0?
1 réponse-
The product may contain a small amount of methemoglobin as described on the specification sheet.
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What is the structure of Product H0267, Hemoglobin A0?
1 réponse-
Hemoglobin A0 is the major hemoglobin protein of adult hemoglobin. It is a tetrameric protein consisting of 2 alpha and 2 beta chains, each of which contains a heme moiety and iron.
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Can Product H0267, Hemoglobin A0, be used in functional studies involving the binding and release of oxygen as a ligand?
1 réponse-
Sigma has not validated this hemoglobin for use in functional studies. The iron in the heme moiety is in the +2 state. However, this does not mean that this hemoglobin will behave necessarily like native hemoglobin nor be useful for functional studies of the molecule.
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How can Product H0267, Hemoglobin A0, be dissolved?
1 réponse-
Hemoglobin is soluble in water at 20 mg/mL. Solutions in phosphate buffer at about neutral pH can also be prepared.
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What is the Department of Transportation shipping information for this product?
1 réponse-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight of H0267 Hemoglobin A0?
1 réponse-
Sigma has not evaluated the molecular weight of hemoglobin. Based on the reported sequence and including the weight of the 4 heme groups and 4 iron atoms, the molecular weight is approximately 64,677 Daltons. The Structure and Action of Proteins, p. 52, Harper and Row (1969).
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