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GE28-9574-99

TALON® Superflow™

Cytiva 28-9574-99, pack of 10 mL

Synonyme(s) :

TALON Affinity Resin

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About This Item

Code UNSPSC :
23151817
Nomenclature NACRES :
NA.56

ligand

TALON® Superflow Cobalt-IMAC

Conditionnement

pack of 10 mL

Fabricant/nom de marque

Cytiva 28-9574-99

Matrice

6% cross-linked agarose

Taille des particules

60-160 μm

cleaning in place

2-14(Co2+-stripped medium)

working range

3-12(Co2+-stripped medium)

Capacité

≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)

Température de stockage

2-8°C

Description générale

Talon® Superflow is a cobalt-based IMAC medium offering different selectivity compared to nickel-charged media.

Caractéristiques et avantages

  • Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant Proteins by immobilized metal affinity chromatography (IMAC).
  • Suitable for IMAC purification of histidine-tagged Proteins when Ni2+ is not the optimal choice of metal ion.
  • Compatible with commonly used IMAC reagents and appropriate for purifying Proteins under native or denaturing conditions.
  • Allows for Protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Superflow is a trademark of Sterogene Bioseparations Inc.
Talon is a registered trademark of Clontech

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Manual Purification of Histidine-Tagged Proteins using His GraviTrap™ TALON® Columns

His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins.

Cell Lysis of Histidine-Tagged Proteins

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Optimizing Purification of Histidine-Tagged Proteins

How to optimize purification of histidine-tagged proteins using Cytiva products.

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

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Protocoles

Purification of Histidine-Tagged Proteins using TALON® Superflow

This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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