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GE17-5053-01

HiTrap® Q Fast Flow

Cytiva 17-5053-01, pack of 5 × 1 mL

Synonyme(s) :

Ion exchange chromatography column

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About This Item

Code UNSPSC :
23151816
Nomenclature NACRES :
NA.56

ligand

quaternary amine

Description

Ion Exchanger Type (value)

Conditionnement

pack of 5 × 1 mL

Fabricant/nom de marque

Cytiva 17-5053-01

Paramètres

<4 mL/min flow rate
42 psi

Taille du lit

7 mm × 25 mm

Volume du lit

1 mL

D.I. de la colonne

7 mm

Matrice

6% cross-linked agarose

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning

2-14

working range

2-12

Adéquation

suitable for bioprocess medium

Description générale

HiTrap® Q FF is prepacked with Q Sepharose™ Fast Flow, and is a strong anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

Q, SP, DEAE, and CM Sepharose™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.

Caractéristiques et avantages

  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  • The industry standard for ion exchange chromatography.
  • High flow rates and good scale-up potential.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Predictable scale-up

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Purifcation or Removal of Serine Proteases, e.g. Thrombin and Trypsin, and Zymogens

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Protocoles

Performing a Purification with Calmodulin Sepharose 4B

This page shows how to purify ATPases, adenylate cyclases, protein kinases, phosphodiesterases and neurotransmitters by affinity chromatography using Calmodulin Sepharose 4B from Cytiva.

Refolding: Ion Exchange Chromatography

Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

This page covers the use of Sepharose Fast Flow for purification of proteins.

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