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G9136

Sigma-Aldrich

Porcine Gelatin

from porcine skin, Type A, powder, γ-irradiated, suitable for cell culture, BioXtra

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About This Item

Numéro CAS:
9000-70-8
Numéro CE :
232-554-6
Numéro MDL:
MFCD00081638
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.75

product name

Gelatin from porcine skin, Type A, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

Source biologique

Porcine skin

Niveau de qualité

200

Stérilité

γ-irradiated

Type

Type A

Gamme de produits

BioXtra

Forme

lyophilized powder

Conditionnement

pkg of 10 mg

Technique(s)

cell culture | mammalian: suitable

Couverture de surface

100‑200 μg/cm2

Résistance du gel

~300 g Bloom

Solubilité

soluble 20 mg/mL, clear, colorless (in warm tissue culture medium)

Conditions d'expédition

ambient

Température de stockage

room temp

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Catégories apparentées

Application

This product is recommended for use as a cell culture substratum at 100-200 μg/cm2. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

Composants

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Attention

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Notes préparatoires

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g.

This product is a type A lyophilized powder, suitable for cell culture, with a gel strength of 300.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Sonia Capellero et al.
International journal of molecular sciences, 23(2) (2022-01-22)
Peritoneal metastases are the leading cause of morbidity and mortality in ovarian cancer. Cancer cells float in peritoneal fluid, named ascites, together with a definitely higher number of non neo-neoplastic cells, as single cells or multicellular aggregates. The aim of
Giulia Villari et al.
The EMBO journal, 39(24), e103661-e103661 (2020-11-21)
Although subcellular positioning of endosomes significantly impacts on their functions, the molecular mechanisms governing the different steady-state distribution of early endosomes (EEs) and late endosomes (LEs)/lysosomes (LYs) in peripheral and perinuclear eukaryotic cell areas, respectively, are still unsolved. We unveil
Marta Czernik et al.
Nature protocols, 11(11), 2170-2188 (2016-10-07)
This protocol describes how to convert the chromatin structure of sheep and mouse somatic cells into spermatid-like nuclei through the heterologous expression of the protamine 1 gene (Prm1). Furthermore, we also provide step-by-step instructions for somatic cell nuclear transfer (SCNT)
Sandeep Kadekar et al.
Cellular and molecular bioengineering, 14(1), 121-130 (2021-02-27)
Human mesenchymal stem cells (hMSCs) have a great clinical potential for tissue regeneration purposes due to its multilineage capability. Previous studies have reported that a single addition of 5-azacytidine (5-AzaC) causes the differentiation of hMSCs towards a myocardial lineage. The
Carla Luana Dinardo et al.
PloS one, 10(12), e0145062-e0145062 (2015-12-15)
Recent evidence shows the rigidity of vascular smooth muscle cells (VSMC) contributes to vascular mechanics. Arterial rigidity is an independent cardiovascular risk factor whose associated modifications in VSMC viscoelasticity have never been investigated. This study's objective was to evaluate if
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