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G8644

Sigma-Aldrich

D-(+)-Glucose solution

100 g/L in H2O, sterile-filtered, BioXtra, suitable for cell culture

Synonyme(s) :

D-Glucopyranose

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About This Item

Formule empirique (notation de Hill) :
C6H12O6
Numéro CAS:
50-99-7
Poids moléculaire :
180.16
Beilstein:
1281608
Numéro MDL:
MFCD00063774
Code UNSPSC :
12352201
ID de substance PubChem :
24895346
Nomenclature NACRES :
NA.75
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Stérilité

sterile-filtered

Niveau de qualité

400

Gamme de produits

BioXtra

Forme

solution

Concentration

100 g/L in H2O

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Température de stockage

room temp

Chaîne SMILES 

OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5-,6+/m1/s1

Clé InChI

WQZGKKKJIJFFOK-DVKNGEFBSA-N

Informations sur le gène

human ... PYGM(5837)

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Catégories apparentées

Description générale

Glucose provides the primary energy source for cell metabolism. It is a main source of energy for living organisms. Glucose occurs naturally in the free state in fruits and other parts of plants. It is combined into glucosides, disaccharides, oligosaccharides, the polysaccharides (cellulose and starch), and glycogen.

Application

D-(+)-Glucose solution has been used:

  • to determine extracellular acidification rate (ECAR) or oxygen consumption rate (OCR) in human CB CD34+cells by Seahorse extracellular flux assay[1]
  • as a negative control in human umbilical vein endothelial cell (HUVEC) culture[2]
  • as a supplement for culturing colonic biopsies[3]

Actions biochimiques/physiologiques

Glucose is an aldosic monosaccharide and acts as an important component of glycolysis and downstream pathways for aerobic and anaerobic respiration. It provides energy for growth and reproduction. Glucose is a byproduct of photosynthesis that is condensed to starch in plants and cyanobacteria.[4]

Autres remarques

This product has been tested with cell lines to verify the product is not cytotoxic. The growth promoting capacity of this cell culture tested glucose solution was assessed at 45 mL/L in medium using appropriate cell lines.
This solution has been sterilized by filtration.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
RNBL7494
RNBL1792
RNBK9929
RNBK3918
RNBJ8362

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Shu J Lam et al.
ACS applied materials & interfaces, 8(49), 33446-33456 (2016-12-15)
'Structurally nanoengineered antimicrobial peptide polymers' (SNAPPs), in the form of star-shaped peptide polymer nanoparticles, have been recently demonstrated as a new class of antimicrobial agents with superior in vitro and in vivo efficacy against Gram-negative pathogens, including multidrug-resistant species. Herein
Qianqian Li et al.
Journal of cellular and molecular medicine, 24(1), 910-920 (2019-11-05)
In type 1 and type 2 diabetes mellitus, increased cardiac fibrosis, stiffness and associated diastolic dysfunction may be the earliest pathological phenomena in diabetic cardiomyopathy. Endothelial-mesenchymal transition (EndMT) in endothelia cells (ECs) is a critical cellular phenomenon that increases cardiac
Brandon Mapes et al.
Physiological genomics, 46(8), 302-308 (2014-02-20)
1α,25-Dihydroxyvitamin D3 [1α,25(OH)2D3] is a steroid hormone derived from circulating 25(OH) vitamin D [25(OH)D] with chemopreventive effects in colorectal cancer. 1α,25(OH)2D3 acts through transcriptional mechanisms; however, our understanding of vitamin D transcriptional responses in the colon is derived from studies
Dieter Henrik Heiland et al.
Nature communications, 10(1), 2541-2541 (2019-06-13)
Reactive astrocytes evolve after brain injury, inflammatory and degenerative diseases, whereby they undergo transcriptomic re-programming. In malignant brain tumors, their function and crosstalk to other components of the environment is poorly understood. Here we report a distinct transcriptional phenotype of
Andreia V Pinho et al.
PloS one, 10(6), e0128012-e0128012 (2015-06-06)
Sirtuin 1 (Sirt1) has been reported to be a critical positive regulator of glucose-stimulated insulin secretion in pancreatic beta-cells. The effects on islet cells and blood glucose levels when Sirt1 is deleted specifically in the pancreas are still unclear. This
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Questions

1–5 sur 5 questions  

  1. Hello what is the recommended concentrations of D-glucose solution 100g/l to used for primary cell culture of brain ?

    1 réponse

    1. There are a number of cell types present in brain tissue. Glucose requirements may vary by cell type. This product is not tested for use in primary cell culture. However, the typical glucose concentration in most classical media is 1 g/L or 5 g/L. In brain slice or hippocampal slice studies, the glucose concentration may vary broadly. It is advisable to search to the literature for the appropriate concentration of D-glucose to use for primary brain cell culture. Please see the links below to review examples of these publications:

      'Organotypic hippocampal slice culture from the adult mouse brain: A versatile tool for translational neuropsychopharmacology'
      https://www.sciencedirect.com/science/article/pii/S0278584612002862?via%3Dihub

      'Preparation of organotypic brain slice cultures for the study of Alzheimer’s disease'
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5964634/

      Utile ?

  2. What is the Molar concentration of Product G8769, D-(+)-Glucose solution?

    1 réponse

    1. The molar concentration of Glucose solutions G8644 and G8769, may only be estimated. The glucose molecular weight is 180.16 g/mol, however the concentration is lot specific.

      Product G8644 has a concentration specification ranging from 90 - 110 g/L or 0.50 - 0.61 M.

      Please see the link below to access a sample or lot-specific Certificate of Analysis:
      https://www.sigmaaldrich.com/product/sigma/g8644#product-documentation

      Product G8769 has a concentration specification ranging from 405 - 495 g/L or 2.24 - 2.74 M.

      Please see the link below to access a sample or lot-specific Certificate of Analysis:
      https://www.sigmaaldrich.com/product/sigma/g8769#product-documentation

      Utile ?

  3. How does the storage temperature relate to shipping conditions?

    1 réponse

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  4. What is the Molar concentration of Product G8644, D-(+)-Glucose solution?

    1 réponse

    1. Based on a 10% (w/v) concentration and a molecular weight for glucose of 180 Da,  Product G8644 D-(+)-Glucose solution, is 0.56 M glucose.

      Utile ?

  5. What is the Department of Transportation shipping information for this product?

    1 réponse

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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