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G5080

Sigma-Aldrich

Sephadex® G-50

Fine

Synonyme(s) :

Gel filtration resin, Sephadex G-50 Fine Medium

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10 G
139,00 €
50 G
391,00 €

139,00 €

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10 G
139,00 €
50 G
391,00 €

About This Item

Numéro CAS:
9048-71-9
Numéro MDL:
MFCD00132771
Code UNSPSC :
23151817
Nomenclature NACRES :
NA.56

139,00 €

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Technique(s)

affinity chromatography: suitable

Groupe de la matrice active

phase

Gonflement

1 g swells to 9-11 mL

Taille des billes

20-80 μm

Application(s)

life science and biopharma

Compatibilité

Cytiva

Catégories apparentées

Description générale

Sephadex G-50 Fine is a widely used gel filtration resin designed for desalting and buffer exchange of biomolecules with a molecular weight greater than 30,000. Its small bead size contributes to higher efficiency in these processes. Different types of Sephadex vary in terms of cross-linking, resulting in differences in swelling and molecular fractionation range. Sephadex G-50 is one of five G-types available, ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 comes in four different particle sizes, such as Coarse, Medium, Fine, and Superfine.
Sephadex® G-50 is a gel filtration medium used in affinity chromatography, protein chromatography, and gel filtration chromatography. Sephadex is a beaded gel prepared by crosslinking dextran with epichlorohydrin.
Fractionation Range (MW)
Globular Proteins: 1,500 - 30,000
Dextrans: 500 - 10,000

Application

Sephadex® G-50 has been used:
  • to remove the non-entrapped carboxyfluorescein (CF) from the liposome suspension[1]
  • in the purification of monoclonal antibody (mAb) humanized MN-14 by centrifuged size-exclusion chromatography[2]
  • in desalting the recombinant enzymes eluted in the fraction five and six[3]

Sephadex® G-50 is suitable for use in:

  • the separation of low and high molecular weight molecules
  • affinity chromatography, protein chromatography, and gel filtration chromatography

Caractéristiques et avantages

  • Desalts, removes contaminants, and transfers to a new buffer in one step.
  • Suitable for DNA purification from small molecules using gel filtration.
  • Features a small bead size, resulting in shorter diffusion distances.
  • Considered a classic gel filtration resin.

  • Desalting with Sephadex is considered superior to dialysis because it saves time, has a low dilution factor, and recovers high activity even with minute amounts of sample

Autres remarques

G5080-100G′s updated product number is GE17-0042-01
G5080-500G′s updated product number is GE17-0042-02

Informations légales

Sephadex is a registered trademark of Cytiva

Remplacé(e)(s) par

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000413766
0000413766
0000411205
0000411205
0000398283

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Relaxation of tyrosine pathway regulation underlies the evolution of betalain pigmentation in Caryophyllales
Lopez-Nieves S, et al.
The New phytologist, 217, 896-908 (2018)
L M Weiner et al.
Journal of immunology (Baltimore, Md. : 1950), 151(5), 2877-2886 (1993-09-01)
Bispecific monoclonal antibodies (BsmAb) with specificity for tumor Ag and effector cell trigger molecules have been shown to redirect the cytotoxicity of several peripheral blood mononuclear cell populations against relevant tumor. The BsmAb, 2B1, binds to the extracellular domain of
L Zhang et al.
The EMBO journal, 14(2), 313-320 (1995-01-16)
Heme is a prosthetic group for numerous enzymes, cytochromes and globins, and it binds tightly, sometimes covalently, to these proteins. Interestingly, heme also potentiates binding of the yeast transcriptional activator HAP1 to DNA and inhibits mitochondrial import of the mammalian
Clinical-scale radiolabeling of a humanized anticarcinoembryonic antigen monoclonal antibody, hMN-14, with residualizing 131I for use in radioimmunotherapy
Govindan SV, et al.
Journal of Nuclear Medicine, 46(1), 153-159 (2005)
Samuel Lopez-Nieves et al.
The New phytologist, 217(2), 896-908 (2017-10-11)
Diverse natural products are synthesized in plants by specialized metabolic enzymes, which are often lineage-specific and derived from gene duplication followed by functional divergence. However, little is known about the contribution of primary metabolism to the evolution of specialized metabolic
Afficher tous les articles scientifiques apparentés

Questions

1–8 sur 8 questions  

  1. What concentration of non-ionic detergent should be used for cleaning of G50 resins

    1 réponse

    1. The specific concentration of non-ionic detergent for cleaning Sephadex Media is not explicitly mentioned in the available data. However, various sources suggest concentrations ranging from 0.1 – 0.5%. This information has not been validated. The general cleaning recommendations are as follows:
      1. Wash with two column volumes of 0.2 M NaOH or a solution of a nonionic detergent. Washing the more porous Sephadex types (G-50 – G-200) with NaOH should be done outside the column because the gel will swell.
      2. Re-equilibrate the gel with 2–3 column volumes of buffer before your next experiment. When necessary, the gel can be removed from the column and sterilized by autoclaving at 120 °C, pH 7.

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  2. Can I use this for spin columns?

    1 réponse

    1. Yes, this product can be used for spin columns. For a prepared version, please view the Roche Quick Spin columns. See the link below to review this product:
      https://www.sigmaaldrich.com/product/roche/11274015001

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  3. What kind of separation can I expect from Sephadex®  G-50, Product G5080?

    1 réponse

    1. As a general rule of thumb, Sephadex® G-50 is appropriate for the separation of molecules of ~30 kDa from molecules ~1.5 kDa

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  4. What is the Department of Transportation shipping information for this product?

    1 réponse

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  5. What is the best buffer to use with Sephadex®  G-50, Product G5080?

    1 réponse

    1. Sephadex® G-50 is very flexible in the buffers that can be used with it.  Essentially any common aqueous buffer can be used with Sephadex® G-50.  It is advised to filter buffers through a 0.22 micron filter before use.

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  6. How can I clean the Sephadex®  G-50, Product G5080, after use?

    1 réponse

    1. To clean the gel, you can wash the gel with 2 column volumes (CV) of 0.2 M NaOH, outside of the column because the gel will swell in basic pH, or in a solution of non-ionic detergent.  Then you can rinse with water and re-equilibrate with 2-3 CV of buffer. If the resin is to be stored (longer than ~24 hours), equilibrate the resin with buffer containing an antimicrobial such as 20% ethanol or 0.02% sodium azide.

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  7. How stable is Sephadex® G-50, Product G5080, after swelling?

    1 réponse

    1. Sephadex® is stable in water, salt solutions, organic and denaturing solvents.  If necessary, the gel can be removed from the column and sterilized by autoclaving at 120 deg C, pH 7. The pH stability is limited to low strengths and short times at extremes of pH 2-10, particularly in the acid range.  After any washes using solutions at extremes of acidic or basic pH, the Sephadex®  should be washed and reequilibrated with buffer at neutral pH as promptly as possible.

      Utile ?

  8. What does "G-50" mean in Sephadex®  G-50, Product G5080?

    1 réponse

    1. "G" stands for gel.  "50" refers to the water regain of the gel, in this instance, 5.0 g water per g dry gel.

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