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FLE250

Firefly Lantern Extract

Name: Firefly Lantern Extract
Brand: SIGMA

for ATP determination

Synonyme(s) :

FLE

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A propos de cet article

NACRES:

NA.81

UNSPSC Code:

12352202

Form:

solution

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Propriétés

Quality Segment

200

form

solution

packaging

pkg of 250 mg dried lanterns

technique(s)

toxicology assay: suitable

storage temp.

−20°C

Description

General description

The bioluminescent organism, Photinus pyralis firefly is the source of firefly lantern extract. The luciferase enzyme is 60 kDa enzyme which lacks prosthetic group and bound metal in its structure. The N-terminal harbors residues crucial for the bioluminescent activity and the C-terminal possess peroxisome targeting motif.

Application

Firefly Lantern Extract has been used in the detection of ATP concentrations by bioluminescence flash analysis in seawater samples, bioluminescence assay in river sediments samples, marine sediments and in quantifying extracellular ATP in red blood cells.

Fireflies have been used in several studies of bioluminescence. In particular they may be used in assays to determine toxicity pathways because bioluminescence can quantitatively measure a wide range of biological activities.

Biochem/physiol Actions

Firefly Lantern Extract containing luciferase catalyzes oxidative decarboxylation of luciferin (LH2) in the presence of ATP, oxygen and Mg²⁺ to yield a light-emitter oxyluciferin.

Preparation Note

After reconstitution with 25 mL of water, vial will contain 0.06 M potassium phosphate, pH 7.4, and 0.02 M MgSO₄^.

Other Notes

Crude luciferin and luciferase

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Cet article	F6303	L9420	SCT150
Sigma-Aldrich FLE250 Firefly Lantern Extract	Sigma-Aldrich F6303 Firefly Lantern Extract	Sigma-Aldrich L9420 Luciferase from Photinus pyralis (firefly)	Sigma-Aldrich SCT150 Firefly Luciferase HTS Assay
description for ATP determination	description pkg of 100 mg	description recombinant, expressed in E. coli, buffered aqueous solution, ≥10×10¹⁰ units/mg protein	description Glow-type luciferase assay for the quantification of firefly luciferase gene expression in mammalian cells with signal half-life of about 3 hours
technique(s) toxicology assay: suitable	technique(s) toxicology assay: suitable	technique(s) -	technique(s) cell based assay: suitable
form solution	form solid	form buffered aqueous solution	form -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. -65 to -96°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level -
packaging pkg of 250 mg dried lanterns	packaging pkg of 100 mg	packaging -	packaging -

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Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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