The low value observed in the sample may be due to several factors. Since the standard curve is working well, it is likely that the lysis buffer and/or homogenization condition used is not optimized for assay reading. To verify the possible presence of inhibiting substances, one could add a known amount of ATP from a standard solution to the homogenate to see if the reading increases accordingly. It's possible that the lysis buffer used was not optimal to yield an adequate amount of ATP for detection. Currently, there is no recommended cardiac tissue preparation protocol for use with this kit. As the kit measures only soluble ATP, it is highly recommended to clarify the homogenized extract by centrifugation to obtain a supernatant solution for use with this assay.
FLAAB
Adenosine 5′-triphosphate (ATP) assay mix dilution buffer
lyophilized powder
Synonyme(s) :
ATP Assay Dilution Buffer, Assay Mix Buffer, Assay Mix for ATP
Sélectionner une taille de conditionnement
| Taille/Référence | Disponibilité | Prix |
|---|---|---|
1 vial | Vérifier la disponibilité des articles du panier | 83,70 € |
5 vials | Vérifier la disponibilité des articles du panier | 437,00 € |
10 vials | Vérifier la disponibilité des articles du panier | 449,00 € |
A propos de cet article
83,70 €
General description
Application
Preparation Note
1 of 1
Cet article | |||
|---|---|---|---|
| description lyophilized powder | description lyophilized powder | description vial of ~1 mg ATP | description Grade I, ≥99%, from microbial |
| technique(s) activity assay: suitable | technique(s) PCR: suitable | technique(s) - | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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It seems that you are currently trying to perform the ATP assay (Sigma Cat# FLAA) on cardiac tissue. The kit appears to be working, as your standard curve is functioning, but you are obtaining very low numbers for your samples, significantly below your standard curve. Given that the heart should contain an abundance of ATP, you are concerned that your extraction protocol might be the issue. You are wondering if there is an extraction protocol available for this tissue for the mentioned kit.
1 réponse-
Utile ?
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