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FD10S

Sigma-Aldrich

Isothiocyanate-dextrane marqué à la fluorescence

average mol wt 10,000

Synonyme(s) :

FITC-dextrane

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About This Item

Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352201
Nomenclature NACRES :
NA.25

Source biologique

sugar beets root

Conjugué

FITC conjugate

Forme

powder

Poids mol.

average mol wt 10,000

Ampleur du marquage

0.003-0.020 mol FITC per mol glucose

Couleur

yellow to yellow-orange

Solubilité

water: 25 mg/mL, cloudy to hazy, yellow to orange

Température de stockage

2-8°C

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Application

Dextrane marqué à l′isothiocyanate de fluorescéine pour utilisation potentielle dans les études de perfusion chez l′animal.

Notes préparatoires

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Autres remarques

Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.
To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Antonini, E. et al.
Biopolymers null
Clotilde Cadart et al.
Nature communications, 9(1), 3275-3275 (2018-08-18)
Despite decades of research, how mammalian cell size is controlled remains unclear because of the difficulty of directly measuring growth at the single-cell level. Here we report direct measurements of single-cell volumes over entire cell cycles on various mammalian cell
The structure of NRRL B-512F dextran, methylation studies.
Van Cleve JW, Schacfer WC and Rist CE
Journal of the American Chemical Society, 78(17), 4435-4438 (1956)
Fluorescein-labelled dextran measurement in interstitial fluid in studies of macromolecular permeability.
G Rutili et al.
Microvascular research, 12(2), 221-230 (1976-09-01)
Anna Marchetti et al.
BMC research notes, 2, 7-7 (2009-01-14)
Dictyostelium amoebae are frequently used to study the organization and function of the endocytic pathway, and specific protocols are essential to measure the dynamics of endocytic compartments and their internal pH. We have revisited these classical protocols to measure more

Contenu apparenté

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.

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