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Merck

FA20

Fructose Assay Kit

sufficient for 20 assays

Synonyme(s) :

Fructose Test Kit

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A propos de cet article

UNSPSC Code:
12164500
EC Number:
200-333-3
NACRES:
NA.84
Technique(s):
photometry: suitable
Usage:
sufficient for 20 assays
Storage temp.:
2-8°C

Principaux documents

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usage

sufficient for 20 assays

Quality Level

100

analyte chemical class(es)

sugars (fructose)

technique(s)

photometry: suitable

application(s)

food and beverages
general analytical

storage temp.

2-8°C

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SA20G3285A4582
Fructose Assay Kit sufficient for 20 assays

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Fructose Assay Kit

Starch (HK) Assay Kit sufficient for 20 assays

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SA20

Starch (HK) Assay Kit

D-(+)-Glucose solution 1 mg/mL in 0.1% benzoic acid, standard for enzymatic assay kits GAGO20, GAHK20, STA20, analytical standard

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G3285

D-(+)-Glucose solution

α-Amylase from Bacillus licheniformis suitable for determination of starch (Kit STA-20)

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A4582

α-Amylase from Bacillus licheniformis

usage

sufficient for 20 assays

usage

sufficient for 20 assays

usage

-

usage

-

technique(s)

photometry: suitable

technique(s)

photometry: suitable

technique(s)

photometry: suitable

technique(s)

photometry: suitable

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

analyte chemical class(es)

sugars (fructose)

analyte chemical class(es)

sugars

analyte chemical class(es)

sugars (glucose)

analyte chemical class(es)

sugars

microbialanalysis
microbialanalysis

microbialanalysis
microbialanalysis

microbialanalysis
microbialanalysis

microbialanalysis
microbialanalysis

Application

This kit is for the quantitative, enzymatic determination of fructose in food and other materials.Fructose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Fructose 6-phosphate is converted to glucose 6-phosphate by phosphoglucose isomerase (PGI). Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in the reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to fructose concentration.

Composants de kit seuls

Réf. du produit
Description
  • fructose standard 500 mg
  • glucose assay reagent 1 mL/vial
  • phosphoglucose isomerase 1 mL/vial

related product

Réf. du produit
Description
Tarif

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

wgk

WGK 3

Classe de stockage

11 - Combustible Solids

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Certificats d'analyse (COA)

Lot/Batch Number
0000524122
0000524122
0000497918
0000497918
0000423000

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Official Methods of Analysis of the AOAC, 28-28 (1995)
Yifan Chen et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 36(3), 373-378 (2019-11-19)
Cholesteryl ester (CE) is an ester of cholesterol and fatty acid (FA). Plasma CE reflects complicated metabolisms of cholesterol, phospholipids, lipoproteins, and dietary FAs. An informatics approach could be useful for analysis of the CE species. In this study, two
Mengxuan Fang et al.
Analytical and bioanalytical chemistry, 412(10), 2339-2351 (2020-02-02)
Fatty acids are a major source of structural diversity within the lipidome due to variations in their acyl chain lengths, branching, and cyclization, as well as the number, position, and stereochemistry of double bonds within their mono- and poly-unsaturated species.
Chih-Yu Cheng et al.
FEBS letters, 589(20 Pt B), 3107-3112 (2015-09-04)
Laccase and borate-fructose complex were investigated by coincidence in a solid-state fermentation of Edenia sp. TS-76 under fructose oxidase screening. Laccase was purified to homogeneity with a 34-fold purification and 32% yield. Fructose had no significant effect on laccase activity
Beuter, H.O.
Methods of Enzymatic Analysis, 6, 321-321 (1985)
Afficher tous les articles scientifiques apparentés

Contenu apparenté

Numéro d'article de commerce international

RéférenceGTIN
FA20-1KT04061833622292
G3293-50ML04061837904998
G3293-20ML04061837904981
F2793-500MG04061833613313

Questions

1–3 sur 3 questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 réponse

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 réponse

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. イネの根および葉のフルクトース含量を測定したいと思っています。こちらのキットを使えるでしょうか。また、商品の詳細（分析手順等）について教えていただけると幸いです。

    1 réponse

    1. Yes, this kit would be suitable. Here is the link to the Technical Bulletin that describes the use of this product:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/111/939/fa20bul.pdf
      For roots and leaves, one would follow the Sample Preparation protocol for Solids. The plant tissue would need to be macerated in order to completely extract the Fructose into the water.

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