F9302
Factor X Activated (Xa) from bovine plasma
aqueous glycerol solution
Synonyme(s) :
Factor Xa
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A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
Numéro CE :
MDL number:
Specific activity:
≥50 units/mg protein
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aqueous glycerol solution
Quality Segment
specific activity
≥50 units/mg protein
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
cow ... F10(280787)
General description
Factor Xa is a serine endoproteinase and a member of the S1 peptidase family. Factor Xa plays a critical role in the coagulation cascade by catalyzing the proteolytic conversion of prothrombin to active thrombin. Factor Xa′s prothrombin conversion activity is greatly enhanced in vivo when complexed with factor V, calcium ions and phospholipids on the activated platelet surface.
The zymogen form, Factor X, is activated in vivo by two different pathways. The intrinsic pathway utilizes a catalytic complex composed of factor IXa, factor VIII, phospholipids and calcium ions. The extrinsic pathway utilizes a complex of factor VII and tissue factor. The factor X zymogen is a 55 KDa glycoprotein with a light and heavy chain joined by a single disulfide.
The zymogen form, Factor X, is activated in vivo by two different pathways. The intrinsic pathway utilizes a catalytic complex composed of factor IXa, factor VIII, phospholipids and calcium ions. The extrinsic pathway utilizes a complex of factor VII and tissue factor. The factor X zymogen is a 55 KDa glycoprotein with a light and heavy chain joined by a single disulfide.
Application
Bovine Factor Xa is used for site specific cleavage of recombinant fusion proteins containing an accessible Factor Xa recognition site for removal of affinity tags.
Fusion proteins are commonly expressed with a factor Xa cleavable Ile-Glu (or Asp)-Gly-Arg-↓-X sequence. Typically 1 mg of fusion protein can be incubated with 10 μg of factor Xa for 2.5 hours at 37 °C.
Biochem/physiol Actions
Factor Xa catalyzes the hydrolysis of the Arg-Thr and then Arg-Ile bonds in prothrombin to yield active thrombin.
The fairly strict recognition sequence is Ile-Glu (or Asp)-Gly-Arg-↓-X.
It may sometimes cleave at other basic residues, depending on the conformation of the target protein. Factor Xa will not cleave if a proline residue follows the arginine of the recognition sequence.
pH Optimum: 7.6-8.0
Temperature Optimum: 37 °C
The fairly strict recognition sequence is Ile-Glu (or Asp)-Gly-Arg-↓-X.
It may sometimes cleave at other basic residues, depending on the conformation of the target protein. Factor Xa will not cleave if a proline residue follows the arginine of the recognition sequence.
pH Optimum: 7.6-8.0
Temperature Optimum: 37 °C
Other Notes
One unit of activated Factor X will liberate 1.0 μmole of p-nitroanilide from N-benzoyl-L-isoleucyl-L-glutamyl-L-glycyl-L-arginine-p-nitroaniline per minute at pH 8.3 at 37 °C.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Cet article | |||
|---|---|---|---|
| description aqueous glycerol solution | description clone HX-1, purified immunoglobulin, buffered aqueous solution | description clone GT268, affinity isolated antibody | description ≥97.0% (HPLC) |
| Gene Information cow ... F10(280787) | Gene Information human ... F10(2159) | Gene Information human ... F10(2159) | Gene Information - |
| specific activity ≥50 units/mg protein | specific activity - | specific activity - | specific activity - |
| form aqueous glycerol solution | form buffered aqueous solution | form buffered aqueous solution | form powder |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in dry ice | shipped in wet ice | shipped in - |
Classe de stockage
10 - Combustible liquids
Que se passe-t-il ?
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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