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F8758

Sigma-Aldrich

Folic acid

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥97%

Synonyme(s) :

Pterylmonoglutamic acid, PteGlu, Pteroyl-L-glutamic acid, Vitamin M

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5 G
47,60 €
25 G
167,00 €

47,60 €


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5 G
47,60 €
25 G
167,00 €

About This Item

Formule empirique (notation de Hill):
C19H19N7O6
Numéro CAS:
Poids moléculaire :
441.40
Beilstein:
100781
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352205
eCl@ss :
34058014
ID de substance PubChem :
Nomenclature NACRES :
NA.75

47,60 €


En stockDétails


Devis pour commande en gros

Source biologique

synthetic

Niveau de qualité

Gamme de produits

BioReagent

Essai

≥97%

Forme

powder

Poids mol.

Mw 441.40 g/mol

Technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

Couleur

dark yellow

Solubilité

1 M NaOH: soluble 50 mg/mL, clear to very slightly hazy, faintly yellow to dark yellow-orange

Température de stockage

room temp

Chaîne SMILES 

NC(N1)=NC(C2=C1N=CC(CNC3=CC=C(C(N[C@@H](CCC(O)=O)C(O)=O)=O)C=C3)=N2)=O

InChI

1S/C19H19N7O6/c20-19-25-15-14(17(30)26-19)23-11(8-22-15)7-21-10-3-1-9(2-4-10)16(29)24-12(18(31)32)5-6-13(27)28/h1-4,8,12,21H,5-7H2,(H,24,29)(H,27,28)(H,31,32)(H3,20,22,25,26,30)/t12-/m0/s1

Clé InChI

OVBPIULPVIDEAO-LBPRGKRZSA-N

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Description générale

Folic acid (FA)/pterylmonoglutamic acid[1] helps to block neural tube defects (NTDs).[2]

Application

Folic acid has been used:
  • to study the role of vitamins and amino acids on hybridoma growth and monoclonal antibody production[3]
  • in cells and treatments[4]
  • in perforin gene targeting using the CRISPR/Cas gene editing approach[5]

Actions biochimiques/physiologiques

A nutritional delivery form of folate. Folic acid and its derivatives are essential mediators of one-carbon metabolism within cells.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Certificats d'analyse (COA)

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Periconceptional folic acid supplementation and the risk of preterm births in China: a large prospective cohort study
Li Z, et al.
International Journal of Epidemiology, 43(4), 1132-1139 (2014)
Reduced expression of galectin-9 contributes to a poor outcome in colon cancer by inhibiting NK cell chemotaxis partially through the Rho/ROCK1 signaling pathway
Wang Y, et al.
PloS one, 11(3), e0152599-e0152599 (2016)
Bone marrow derived mesenchymal stem cells ameliorate inflammatory response in an in vitro model of familial hemophagocytic lymphohistiocytosis 2
Sevim H, et al.
Stem Cell Research & Therapy, 9(1), 198-198 (2018)
Folic acid handling by the human gut: implications for food fortification and supplementation
Patanwala I, et al.
American Journal of Clinical Nutrition, 100(2), 593-599 (2014)
The role of vitamins and amino acids on hybridoma growth and monoclonal antibody production
Ducommun P, et al.
Cytotechnology, 37(2), 65-73 (2001)

Articles

Explore folic acid importance in serum-free eukaryotic cell cultures, including CHO cells, and its role in media supplementation.

Questions

1–5 sur 5 questions  
  1. Is it better to store folic acid (Product No. F8758) solution at RT or -20'C? Also, how long is it stable as a solution for at recommended temperature?

    1 réponse
    1. Solution stability has not been determined for this product, however commercial and pharmaceutical preparations are cited as being stable at room temperature for up to 18 months, protected from light. Folic acid is also a component of most of the classical DMEM media formulations, for example D5546. These products are also assigned a shelf life of 18 months, but are stored at 2 - 8 deg. C. Storing at -20 deg. C may or may not extend the shelf life. There is a possibility that the freeze/thaw process may result in precipitation and may be detrimental.

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  2. Is Product No. F8758, Folic Acid, tested for endotoxins?

    1 réponse
    1. No, Product No. F8758 has not been tested for endotoxins. This product has, however, been tested for use in mammalian cell culture and insect cell culture.

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  3. What is the Department of Transportation shipping information for this product?

    1 réponse
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. What is the stability of folic acid (Product No. F8758) once in solution?

    1 réponse
    1. This product is photolabile and is inactivated by UV light. Alkaline solutions are reasonably stable in the dark, but heating will destroy both alkaline and acidic solutions.

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  5. What is the solubility of folic acid (Product No. F8758)?

    1 réponse
    1. Product No. F8758 is soluble in 1 M NaOH (50 mg/mL). The free acid is only slightly soluble in water (0.01 mg/mL at 0 °C), and is insoluble in aqueous solutions below pH 5. It is soluble in sodium bicarbonate or sodium hydroxide solutions.

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