F5027

Monoclonal Anti-IgD−FITC antibody produced in mouse

clone HJ9, purified immunoglobulin, buffered aqueous solution

• Synonyme(s) : Monoclonal Anti-Human IgD
• Code UNSPSC : 12352203

Propriétés

• Source biologique: mouse
• Conjugué: FITC conjugate
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: secondary antibodies
• Clone: HJ9, monoclonal
• Forme: buffered aqueous solution
• Espèces réactives: human
• Technique(s): flow cytometry: 10 μL using 1 × 10⁶ cells
• Isotype: IgG1
• Numéro d'accès UniProt: P01880
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... IGHD(3495)

Description

Spécificité

Recognizes the delta chain of human IgD and is not reactive with heavy chains from human IgG1, IgG2, IgG3, IgG4, IgM, IgA, or kappa and lambda light chains.

Immunogène

human IgD

Application

Monoclonal Anti-IgD-FITC antibody is suitable for flow cytometry at a concentration of 10μL using 1×10⁶ cells.

Actions biochimiques/physiologiques

IgD is the major antigen receptor isotype coexpressed with IgM on the surface of human peripheral B cells. It is associated with early antibody response. It occupies approximately 0.25% of total immunoglobulins. In serum, it has shown that the catabolism of IgD is decreased at high concentrations. IgD molecule comprises of a long “hinge” region (between Fab and Fc) including three constant region domains. This makes the molecule very susceptible to proteolytic degradation with production of Fab and Fc fragments. It also helps to maintain the molecule flexibility that enhance antigen binding capacity. IgD is a potent inducer of tumor necrosis factor (TNF), Interlukin 1β(IL1β), and Interlukin IL1RN (IL1RN). It also accelerates the release of IL6, IL10 from peripheral blood mononuclear cells. Its increased level in serum causes hyper-IgD and periodic fever syndrome (HIDS).

Autres remarques

IgD is expressed on peripheral B cells and is used as a pan-B cell marker.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Notes préparatoires

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.