F2518

Human Fibronectin

from human foreskin fibroblasts, powder, suitable for cell culture

• Synonyme(s) : Fibronectin
• Numéro CE : 289-149-2
• Numéro MDL: MFCD00131062
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.75

Propriétés

• product name: Fibronectine, lyophilized powder, suitable for cell culture
• Source biologique: human foreskin fibroblasts
• Niveau de qualité: 200
• Gamme de produits: BioReagent
• Forme: lyophilized powder
• Poids mol.: ~550 kDa
• Conditionnement: pkg of 0.5 mg
• Technique(s): cell culture | mammalian: suitable
• Couverture de surface: 1‑5 μg/cm²
• Impuretés: Human Source, none detected (HCV); Human Source, none detected (HIV); Human Source, none detected (HTLV); Human Source, none detected (HbSAg); Microbial Contamination, passes test
• Solubilité: H₂O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)
• Numéro d'accès NCBI: NM_212482.4
• Numéro d'accès UniProt: P02751
• Spécificité de la liaison: Peptide Source: Collagen
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Informations sur le gène: human ... FN1(2335)

Description

Description générale

Fibronectin 1 (FN1) is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. Fibronectin is mostly synthesized by fibroblasts and endothelial cells.It is an adhesive glycoprotein, predominant in extracellular matrix (ECM) and is widely distributed in tissue and blood. The fibronectin structure consists of blocks of repeating peptide sequences that are homologous and form functional units arranged linearly ontwo subunit arms.

Application

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells

Fibronectin human foreskin fibroblasts has been used in extracellular matrix binding assay and probiglycan and cleavage assays. It has also been used in ectoderm (neuronal) differentiation and in the preparation of a semi-solid growth medium.

Actions biochimiques/physiologiques

Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Fibronectin production by normal human foreskin fibroblasts has been shown to improve two-fold after glucocorticoid treatment.

Caractéristiques et avantages

• Fibronectin is isolated from human foreskin fibroblasts maintained in serum-free medium.
• It can be used as a cell attachment factor for various cell types, including epithelial, mesenchymal, neuronal, neural crest, endothelial cells, and fibroblasts.
• The starting material is tested and found negative for HIV and HBsAg.
• The product is soluble in sterile water, yielding a clear, colorless solution.
• It can be stored at -20°C for at least three years.

Attention

Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.

Forme physique

Lyophilized in CAPS buffered saline

Notes préparatoires

dialyzed against chloroform

Reconstitution

Store reconstituted solution in working aliquots at -20°C or lower.

Remarque sur l'analyse

Protein homogeneity is evaluated by immunoelectrophoresis.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)