The presence of EDTA, SDS, or excess salt can affect my PCR/ sequencing reaction. The lysis buffer information is proprietary, but we can say it is free of chaotropic salts. The resins are desalting resins so EDTA, SDS, and salts are depleted.
EC200
GenElute™-E Single Spin Blood DNA High Yield Kit
Reagents and consumables for liquid blood samples. 10, 50 or 250 purifications.
Caractéristiques en matière de développement durable
Synonyme(s) :
GenElute™-E Blood DNA High Yield Kit, GenElute™-E kits
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A propos de cet article
NACRES:
NA.52
UNSPSC Code:
41105600
Technique(s):
DNA purification: suitable
Storage temp.:
2-8°C
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Service technique
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Laissez-nous vous aiderpurified by
(Single-spin negative chromotography), (Time: 30 minutes or less)
feature
Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For the purification of genomic DNA from liquid blood samples), Typical/expected yield (Varies by sample. Please reference user guide for more information.)
sustainability
Sustainability features
technique(s)
DNA purification: suitable
input
blood (liquid or dried)
test parameters
: 3 min hands on time, sample volume: 200-1000 μL blood
storage temp.
2-8°C
General description
GenElute™-E purification kits provide a simple alternative for DNA purification. Compared with traditional methods, GenElute™-E offers:
Traditional silica-based, bind-wash-elute purification kits require multiple wash steps to remove impurities from the spin columns. These steps increase the risk of cross contamination, subject the DNA to centrifugation sheering forces, and introduce chaotropic salts that can carry over into the final sample, inhibiting downstream applications.
GenElute™-E kits employ size exclusion negative chromatography to separate large nucleic acid molecules from smaller protein, lipid, and ionic components in cell, tissue, blood, and other samples. Single-spin columns efficiently absorb and retain cellular debris and sample contaminants while allowing nucleic acids to pass through, reducing the number of steps and plastic materials required for purification. This novel method for high-quality purification is made possible by our innovative SmartLyse® Protease, which enables fast and efficient lysis of a wide range of sample types.
Isolate nucleic acid in a fraction of time compared to traditional silica bind-wash-elute procedures. Simply mix sample with lysis components and incubate according to kit protocol, pipette sample onto the spin column, and centrifuge directly into a collection tube. Cellular debris and contaminants remain bound in the column to be discarded, while purified DNA is ready to be used in downstream applications or stored.
- Isolation of DNA in under an hour with minimal sample handling
- Better purity, leading to improved performance in PCR and other applications
- Significant reduction in plastic and hazardous chemical waste
Traditional silica-based, bind-wash-elute purification kits require multiple wash steps to remove impurities from the spin columns. These steps increase the risk of cross contamination, subject the DNA to centrifugation sheering forces, and introduce chaotropic salts that can carry over into the final sample, inhibiting downstream applications.
GenElute™-E kits employ size exclusion negative chromatography to separate large nucleic acid molecules from smaller protein, lipid, and ionic components in cell, tissue, blood, and other samples. Single-spin columns efficiently absorb and retain cellular debris and sample contaminants while allowing nucleic acids to pass through, reducing the number of steps and plastic materials required for purification. This novel method for high-quality purification is made possible by our innovative SmartLyse® Protease, which enables fast and efficient lysis of a wide range of sample types.
Isolate nucleic acid in a fraction of time compared to traditional silica bind-wash-elute procedures. Simply mix sample with lysis components and incubate according to kit protocol, pipette sample onto the spin column, and centrifuge directly into a collection tube. Cellular debris and contaminants remain bound in the column to be discarded, while purified DNA is ready to be used in downstream applications or stored.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" and "Designing safer chemicals". Click here for more information.
Application
Store enzymes at 2-8°C for up to 12 months. All other components should be stored at room temperature.
Traditional methods for purifying genomic DNA from liquid blood samples ≥ 200 μl rely on medium and large format silica (bind-wash-elute) purification kits, which suffer from limited binding capacity, low DNA concentration, and low purity. The GenElute™-E Single Spin Blood DNA High Yield Kit, extracts up to 20 μg highly concentrated DNA (200 ng/μl) while offering the flexibility of sample inputs ranging from 200 μl to 1 ml liquid blood. EDTA-, citrate- as well as heparin-stabilized human and animal blood can be used. Enriched DNA yield and concentration make this kit well suited for researchers with limited or low-titer samples who require high sensitivity for NGS or other detection methods.Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Genomic DNA preparations usually show an A260/280 ratio of 1.8 to 2.0.
Features and Benefits
Fast and efficient purification: 10-minute lysis and minimal hands-on, preparation stepsHigher quality DNA: negative chromotography-based purification reduces carryover of PCR inhibitors and other contaminents Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals
Other Notes
We believe that green chemistry will contribute to a better tomorrow. With a growing portfolio of greener alternatives, there are now more choices to reduce the ecological impact of your research while still delivering quality and efficacy so your results are not compromised. GenElute™-E purification kits avoid the use of hazardous wash buffers typically employed in silica-based purification kits, significantly reducing chemical waste. The single-spin protocol also limits the number of collection steps, reducing plastic waste by 55% compared to equivalent bind-wash-elute methods. GenElute™-E is designated a Greener Alternative Product based on the "Prevention" and "Designing Safer Chemicals" qualifications of The 12 Principles of Green Chemistry.GenElute™-E kits also adhere to the principles of SMASH Packaging, our global initiative to improve the sustainability through the reduction of packaging materials, the increased use of sustainable alternatives, and improved recyclability. Packaging is FCS-marked for sustainable forestry practices and produced from greater than 70% recycled content.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
SmartLyse is a registered trademark of Merck KGaA, Darmstadt, Germany
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Cet article | |||
|---|---|---|---|
| description Reagents and consumables for liquid blood samples. 10, 50 or 250 purifications. | description Reagents and consumables for various cell culture sample types. 10, 50 or 250 purifications. | description Reagents and consumables for clean-up of pre-purified DNA. 10, 50 or 250 purifications. | description Reagents and consumables for various tissue sample types. 96-well plate format. |
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. room temp | storage temp. 2-8°C |
| input blood (liquid or dried) | input cells | input - | input - |
| purified by (Time: 30 minutes or less), (Single-spin negative chromotography) | purified by (Single-spin negative chromotography), (Time: 30 minutes or less) | purified by (Single-spin negative chromotography), (Time: 3 minutes or less) | purified by (Single-spin negative chromotography) |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
| test parameters sample volume: 200-1000 μL blood, : 3 min hands on time | test parameters : 3 min hands on time | test parameters sample volume: 90-110 μL, : 3 min hands on time | test parameters - |
Composants de kit seuls
Réf. du produit
Description
- Erythrocyte Lysis Buffer
- Blood Lysis Buffer HY
- SmartLyse® HY Protease
- Clearing Solution HY
- 1x Tris Buffer
- Spin Columns
Mot-clé
Danger
Codes de danger
Mentions de précaution
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Resp. Sens. 1
Classe de stockage
3 - Flammable liquids
Que se passe-t-il ?
WGK 3
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What is the composition of the lysis buffer and clearing buffer after flowing through the resin?
1 réponse-
Utile ?
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Genelute-e single spin DNA and RNA purification kits use negative chromatographty to isolate nucleic acids. Can you explain how this approach simplifies workflows?
1 réponse-
Instead of optimizing the bind, wash, and release steps in conventional silica-based spin purification preps, the technology focuses on a separation by performing a single step fractionation based on the size of the biomolecules, which results in depleted impurities.
Utile ?
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Do we know how stable the purified DNA is through several freeze-thaw cycles?
1 réponse-
This will fluctuate due to sample variability (sample collection, concentration, fragment length, sequence [GC content], storage before isolation, etc.). However, the sample is buffer exchanged into a standard storage buffer that is included in the kit (1X TE).
Utile ?
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Does the technology introduce any bias into the sample?
1 réponse-
GenElute™-E does not introduce biases that some “bind-wash-elute” technologies can add because the technology separates by size, rather than by what binds and what is released.
Utile ?
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