This product is provided as a slurry containing 10 mg of enzyme in saturated ammonium sulfate precipitate. It is supplied at 100 units, with a concentration of 0.13 units per 1 mg of protein at 10 mg per ml.
E6412
Cellobiohydrolase I from Hypocrea jecorina
100 U/mg, recombinant, expressed in corn
Synonyme(s) :
Cel7A, Cellobiosidase, Cellulase
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expressed in corn
Quality Segment
form
liquid
specific activity
100 U/mg
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shipped in
dry ice
storage temp.
−20°C
General description
Cellubiohydrolase I is an enzyme present in many fungi, but particularly wood rot fungi. It is a monomer of 53 kDa with a catalytic domain and a cellulose binding domain. The reaction adds water to the glucose bonds in cellulose (non-reducing ends of the chain), yielding cellobiose.
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Application
Cellobiohydrolase I can be used in combination with endocellulases and b-glucosidase to produce glucose from cellulose.
Biochem/physiol Actions
Cellobiohydrolase (CBH) is a cellulase which degrades cellulose by hydrolysing the 1,4-β-D-glycosidic bonds. CBH is an exocellulase which cleaves two to four units from the ends of cellulose. CBH I cleaves progressively from the reducing end. CBH I is commonly used in detergents for cleaning textiles. Its ezymatic activity ranges from 37° C to 50° C, with its optimal temperature being approximately 45° C. The optimum pH for the enzyme is 5-6.
Physical form
Provided as an ammonium sulfate precipitate with the source as recombinant maize.
Other Notes
Unit Definition: A unit will turn over 1 nmole of methyl-umbelliferyl beta-D cellobioside per min at pH 5 at 50° C.
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Cet article | |||
|---|---|---|---|
| specific activity 100 U/mg | specific activity - | specific activity 0.3-3.0 unit/mg solid (using a β-galactose dehydrogenase system and locust bean gum as substrate) | specific activity ≥700 units/g |
| recombinant expressed in corn | recombinant - | recombinant - | recombinant - |
| form liquid | form powder | form powder | form aqueous solution |
| shipped in dry ice | shipped in wet ice | shipped in - | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe de stockage
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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