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E6157

Sigma-Aldrich

Anti-eIF2Bβ (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-EIF-2B, Anti-EIF2B-beta, Anti-EIF2BB, Anti-Eukaryotic translation initiation factor 2B, beta, Anti-Eukaryotic translation initiation factor 2B, subunit 2

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~39 kDa

Espèces réactives

rat, mouse, human

Concentration

~1.0 mg/mL

Technique(s)

immunoprecipitation (IP): 5-10 μg using K562 cell lysates
western blot: 1-2 μg/mL using K562 cell lysates

Numéro d'accès UniProt

P49770

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... EIF2B2(8892)
mouse ... Eif2b2(217715)
rat ... Eif2b2(84005)

Description générale

Translation initiation factor eIF-2B subunit beta, eIF2B β is a component of the regulatory subcomplex of eIF2B along with the eIF2Bα and -δ subunits. eIF2Bβ gene is mapped to human chromosome 14q24.3.

Spécificité

Anti-eIF2Bβ (C-terminal) specifically recognizes human, mouse, and rat eIF2Bβ.

Application

Anti-eIF2Bβ (C-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 5-10μg using K562 cell lysates and western blotting at a concentration of 1-2μg/mL using K562 cell lysates.
Anti-eIF2Bβ (C-terminal) antibody produced in rabbit may be used in immunoblotting.

Actions biochimiques/physiologiques

The eIF2Bα,-β, and -δ subunits downregulate eIF2B activity in response to the phosphorylation of eIF2 on Ser51. Recent studies have linked inherited mutations in any of the five eIF2B subunits to a fatal human disorder known as childhood ataxia with central nervous system hypomyelination (CACH) or vanishing white matter (VWN) disease.
Translation initiation factor eIF-2B subunit β is a part of a five-subunit complex that catalyzes guanine nucleotide exchange on eIF2. EIF2B2 is associated with the cause of autosomal recessive disorders. Autosomal recessive inherited mutations in each of the five eukaryotic initiation factor 2B (eIF2B) subunits are known to cause white matter abnormalities called eIF2B-related disorders.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

G D Pavitt
Biochemical Society transactions, 33(Pt 6), 1487-1492 (2005-10-26)
eIF2B (eukaryotic initiation factor 2B) is a multisubunit protein that is required for protein synthesis initiation and its regulation in all eukaryotic cells. Mutations in eIF2B have also recently been found to cause a fatal human disease called CACH (childhood
Yukiko Hata et al.
International journal of clinical and experimental pathology, 7(6), 3355-3362 (2014-07-18)
We report a rare autopsy case of early infantile-onset vanishing white matter disease, with a submicroscopic deletion of 14q24.3, which included EIF2B2 and a missense mutation of EIF2B2 (V85E) of the remaining allele. The patient was a 4-year-old boy, who
W Yang et al.
Molecular and cellular biology, 16(11), 6603-6616 (1996-11-01)
Eukaryotic translation initiation factor 2B (eIF2B) is a five-subunit complex that catalyzes guanine nucleotide exchange on eIF2. Phosphorylation of the alpha subunit of eIF2 [creating eIF2(alphaP]) converts eIF2 x GDP from a substrate to an inhibitor of eIF2B. We showed
Andreas Ohlenbusch et al.
Human mutation, 25(4), 411-411 (2005-03-19)
Autosomal recessive inherited mutations in each of the five eukaryotic initiation factor 2B (eIF2B) subunits are known to cause white matter abnormalities with a wide continuum of clinical signs and severity leading to the concept of eIF2B-related disorders. The clinical

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