E5763

Escherichia coli heat-stable enterotoxin STa

lyophilized powder, ~100,000 units/mg protein (HPLC)

• UNSPSC Code: 12352200
• MDL number: MFCD00131029

Propriétés

• form: lyophilized powder
• specific activity: ~100,000 units/mg protein (HPLC)
• mol wt: 1,972 Da
• solubility: aqueous buffer: ≥5 mg/mL (More soluble under acidic conditions)
• storage temp.: −20°C

Description

Biochem/physiol Actions

STa has a tertiary structure, maintained by disulfide bridges, which is required for receptor binding and biological activity. Its receptors are membrane-bound guanylyl cyclases. These receptors are located on enterocytes, colonocytes, and various extraintestinal tissues. STa causes diarrhea in humans by binding to its receptor, stimulating the guanylyl cyclase, and triggering production of cyclic GMP. Endogenous ligands for the STa receptor include guanylin, extracted from intestine, and uroguanylin from urine. These peptides may have a role in the regulation of fluid and electrolytes. Protein kinase C (PKC) phosphorylates and activates the STa receptor/guanylyl cyclase in vitro and in vivo. As a result, stimulators of PKC synergistically enhance STa effects on cGMP and secretion.

Other Notes

E. coli STa is a heat-stable peptide toxin.

One unit is the amount of toxin which produces an intestinal weight/carcass weight ratio of ≥0.083 in 3-day-old mice.

Informations sur la sécurité

• Classe de stockage: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable