E2906
Anti-dEGF Receptor, Extracellular Domain antibody, Mouse monoclonal
clone C-273, purified from hybridoma cell culture
Synonyme(s) :
Anti-Drosophila Epidermal Growth Factor Receptor, Anti-dEGFR
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About This Item
Produits recommandés
Source biologique
mouse
Conjugué
unconjugated
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
C-273, monoclonal
Forme
buffered aqueous solution
Espèces réactives
Drosophila
Concentration
~1.2 mg/mL
Technique(s)
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 0.25-0.5 μg/mL using recombinant protein representing amino acids 299-359 of the Drosophila EGFR extracellular domain
Isotype
IgG1
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
Drosophila melanogaster ... Egfr(37455)
Description générale
Anti-dEGF Receptor, Extracellular Domain antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma C-273 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a recombinant protein encoding at the extracellular portion of Drosophila epidermal growth factor receptor (EGFR). Drosophila EGF receptor, also known as Torpedo or DER has four ligands: Gurken, Spitz (the principle ligand), Vein and Argos. Two accessory proteins modulate its signalling: Rhomboid and Star.
Application
Suitable for the following:
- Immunohistochemistry
- Indirect ELISA
- Western blotting at a concentration of 0.25-0.5μg/mL using recombinant protein representing amino acids 299-359 of the Drosophila EGFR extracellular domain
Actions biochimiques/physiologiques
Drosophila epidermal growth factor receptor (EGFR) is involved in many developmental processes such as: egg polarity, cell identity in the ventral ectoderm, neurogenesis, development of the Malpighian tubules, and larval eye and wing development. The downstream signalling molecules that are activated by the Drosophila EGF receptor include: Src homology (Shc), DRK (a homolog of mammalian Grb2), a guanine nucleotide exchange factor (SOS) activated by DRK, and downstream targets including orthologs of Ras, Raf, and Rolled (MAP Kinase).
The epidermal growth factor receptor (EGFR) binds to its cognate ligands that lead to autophosphorylation of receptor tyrosine kinase and subsequent activation of signal transduction pathways that are involved in regulating cellular proliferation, differentiation and survival. EGFR is overexpressed in tumor cell lines and is associated with poor prognosis leading to decreased survival. Its activation also plays a role in resistance to chemotherapy and radiation treatment in tumor cells. EGFR tyrosine kinase inhibitors (TKIs) may act as a new treatment option for advanced lung adenocarcinoma. Its ligands function in the Drosophila CNS and maintains glial survival. Drosophila EGF receptor plays a critical role during the development of Drosophila retina.
Forme physique
Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Produit(s) apparenté(s)
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Regulating the dynamics of EGF receptor signaling in space and time
Shilo BZ
Development, 132(18), 4017-4027 (2005)
Nicole T Revaitis et al.
Development (Cambridge, England), 147(15) (2020-07-19)
To bridge the gap between qualitative and quantitative analyses of the epidermal growth factor receptor (EGFR) in tissues, we generated an sfGFP-tagged EGF receptor (EGFR-sfGFP) in Drosophila The homozygous fly appears similar to wild type with EGFR expression and activation
Dan Liu et al.
Nature communications, 13(1), 6243-6243 (2022-10-22)
Cell competition is a conserved homeostatic mechanism whereby epithelial cells eliminate neighbors with lower fitness. Cell communication at the interface of wild-type "winner" cells and polarity-deficient (scrib-/-) "losers" is established through Sas-mediated Ptp10D activation in polarity-deficient cells. This tumor-suppressive cell
Roy S Herbst
International journal of radiation oncology, biology, physics, 59(2 Suppl), 21-26 (2004-05-15)
The epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein that constitutes one of four members of the erbB family of tyrosine kinase receptors. Binding of EGFR to its cognate ligands leads to autophosphorylation of receptor tyrosine kinase and subsequent
Rebecca P Kim-Yip et al.
Development (Cambridge, England), 145(23) (2018-11-06)
Adult stem cell niche boundaries must be precisely maintained to facilitate the segregation of stem cell and daughter cell fates. However, the mechanisms that govern this process in epithelial tissues are not fully understood. In this study, we investigated the
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